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Team:Warsaw/Calendar-Main/6 June 2008
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<h3> Blue/white and rifampicin test </h3> | <h3> Blue/white and rifampicin test </h3> | ||
<h4>Michał L., Ewa, Marcin:</h4> | <h4>Michał L., Ewa, Marcin:</h4> | ||
| - | < | + | <p>Making <a href="https://2008.igem.org/wiki/index.php?title=Wiki/Team:Warsaw/protocols#chemocompetent">chemocompetent</a> <i>E. coli</i> <a href="https://2008.igem.org/Wiki/Team:Warsaw/igem_notebook.htm#top10">TOP10</a> carrying <a href="https://2008.igem.org/Wiki/Team:Warsaw/vectors/pZC320">pZC320</a>. </p><br> |
<html><h3>Preparation of pMPMT5-AID+AIDT7 construct</h3> | <html><h3>Preparation of pMPMT5-AID+AIDT7 construct</h3> | ||
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<li><a href="https://2008.igem.org/wiki/index.php?title=Wiki/Team:Warsaw/protocols#plasmid_DNA_isolation">Isolation</a> of hypothetical pMPM-T5-AID+AIDT7.</li> | <li><a href="https://2008.igem.org/wiki/index.php?title=Wiki/Team:Warsaw/protocols#plasmid_DNA_isolation">Isolation</a> of hypothetical pMPM-T5-AID+AIDT7.</li> | ||
<li>Control <a href="https://2008.igem.org/wiki/index.php?title=Wiki/Team:Warsaw/protocols#digest">Digest</a> of pMPM-T5-AID+AIDT7 with BamHI.</li> | <li>Control <a href="https://2008.igem.org/wiki/index.php?title=Wiki/Team:Warsaw/protocols#digest">Digest</a> of pMPM-T5-AID+AIDT7 with BamHI.</li> | ||
| - | <li>DNA gel electrophoresis.</li></ol> | + | <li>DNA gel electrophoresis. Fig.1 .</li></ol> |
</html> | </html> | ||
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Blue/white and rifampicin testMichał L., Ewa, Marcin:Making chemocompetent E. coli TOP10 carrying pZC320. Preparation of pMPMT5-AID+AIDT7 constructMichał K.:
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