Team:Warsaw/Calendar-Main/6 June 2008

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<li>Control <a href="https://2008.igem.org/wiki/index.php?title=Wiki/Team:Warsaw/protocols#digest">Digest</a> of pMPM-T5-AID+AIDT7 with BamHI.</li>
<li>Control <a href="https://2008.igem.org/wiki/index.php?title=Wiki/Team:Warsaw/protocols#digest">Digest</a> of pMPM-T5-AID+AIDT7 with BamHI.</li>
<li>DNA gel electrophoresis. Fig.1 .</li></ol>
<li>DNA gel electrophoresis. Fig.1 .</li></ol>
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<var>Fig. 1. Control digest of the pMPMT5-AID+AIDT7 construct.<br> Lane 1 - 25 cycles, lane 2 - 20 cycles.</var>
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Revision as of 10:32, 11 October 2008

Gallery Bricks Notebook Team Project Home


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Blue/white and rifampicin test

Michał L., Ewa, Marcin

Making chemocompetent E. coli TOP10 carrying pZC320.


Preparation of pMPMT5-AID+AIDT7 construct

Michał K.

  1. Isolation of hypothetical pMPM-T5-AID+AIDT7.
  2. Control Digest of pMPM-T5-AID+AIDT7 with BamHI.
  3. DNA gel electrophoresis. Fig.1 .
Fig. 1. Control digest of the pMPMT5-AID+AIDT7 construct.
Lane 1 - 25 cycles, lane 2 - 20 cycles.

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