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Team:Warsaw/Calendar-Main/6 June 2008
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<h4>Michał K.</h4><ol> | <h4>Michał K.</h4><ol> | ||
<li><a href="https://2008.igem.org/wiki/index.php?title=Wiki/Team:Warsaw/protocols#plasmid_DNA_isolation">Isolation</a> of hypothetical pMPM-T5-AID+AIDT7.</li> | <li><a href="https://2008.igem.org/wiki/index.php?title=Wiki/Team:Warsaw/protocols#plasmid_DNA_isolation">Isolation</a> of hypothetical pMPM-T5-AID+AIDT7.</li> | ||
| - | <li>Control <a href="https://2008.igem.org/wiki/index.php?title=Wiki/Team:Warsaw/protocols#digest">Digest</a> of pMPM-T5-AID+AIDT7 with BamHI.</li> | + | <li>Control <a href="https://2008.igem.org/wiki/index.php?title=Wiki/Team:Warsaw/protocols#digest">Digest</a> of pMPM-T5-AID+AIDT7 with BamHI (BamHI buffer).</li> |
| - | <li>DNA gel electrophoresis. Fig.1 .</li></ol> | + | <li>DNA gel electrophoresis. Fig.1 .</li></ol> <br> |
<img src="https://static.igem.org/mediawiki/2008/3/34/Trawienie_aat_kontrola_04_06_2008.jpg"> | <img src="https://static.igem.org/mediawiki/2008/3/34/Trawienie_aat_kontrola_04_06_2008.jpg"> | ||
| - | <var>Fig. 1. Control digest of the pMPMT5-AID+AIDT7 construct.<br> Lane | + | <var>Fig. 1. Control digest of the pMPMT5-AID+AIDT7 construct.<br> Lane 2 - 5: isolated plasmids digested with BamHI</var> |
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Revision as of 10:34, 11 October 2008
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Blue/white and rifampicin testMichał L., Ewa, MarcinMaking chemocompetent E. coli TOP10 carrying pZC320. Preparation of pMPMT5-AID+AIDT7 constructMichał K.
Fig. 1. Control digest of the pMPMT5-AID+AIDT7 construct.Lane 2 - 5: isolated plasmids digested with BamHI |
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