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Team:Warsaw/Calendar-Main/1 July 2008
From 2008.igem.org
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<h4>Michał L., Ewa, Marcin</h4> | <h4>Michał L., Ewa, Marcin</h4> | ||
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| - | <li>Isolation of plasmids from transformants.</li> | + | <li><a href="https://2008.igem.org/Wiki/Team:Warsaw/protocols#plasmid_DNA_isolation">Isolation</a> of plasmids from transformants.</li> |
<li>Control <a href="https://2008.igem.org/wiki/index.php?title=Wiki/Team:Warsaw/protocols#digest"> digest</a> of plasmids with SacI+NotI (BamHI buffer).</li> | <li>Control <a href="https://2008.igem.org/wiki/index.php?title=Wiki/Team:Warsaw/protocols#digest"> digest</a> of plasmids with SacI+NotI (BamHI buffer).</li> | ||
<li>Gel electrophoresis of digested DNA.</li> | <li>Gel electrophoresis of digested DNA.</li> | ||
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<h3>Preparation of constructs with OmpA protein fusions</h3><h4>Paweł</h4> | <h3>Preparation of constructs with OmpA protein fusions</h3><h4>Paweł</h4> | ||
<p><ol> | <p><ol> | ||
| - | <li> Isolation of pET15b plasmid.</li> | + | <li> <a href="https://2008.igem.org/Wiki/Team:Warsaw/protocols#plasmid_DNA_isolation">Isolation</a> of pET15b plasmid.</li> |
<li> Overnight <a href="https://2008.igem.org/Wiki/Team:Warsaw/igem_notebook.htm#digest">digest</a> of pET15b plasmid with NdeI and BamHI, <a href="https://2008.igem.org/wiki/index.php?title=Wiki/Team:Warsaw/protocols#removing">dephosphorylation with CIAP</a>.</li></ol></p> | <li> Overnight <a href="https://2008.igem.org/Wiki/Team:Warsaw/igem_notebook.htm#digest">digest</a> of pET15b plasmid with NdeI and BamHI, <a href="https://2008.igem.org/wiki/index.php?title=Wiki/Team:Warsaw/protocols#removing">dephosphorylation with CIAP</a>.</li></ol></p> | ||
Revision as of 13:51, 11 October 2008
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Cloning alpha-A and omega-A fusions on pKSMichał L., Ewa, Marcin
We have successfully cloned A-alpha and A-omega fusions on pKS vector. The DNA will be now sequenced to ensure that our constructs are correct. Preparation of constructs with OmpA protein fusionsPaweł
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