Alberta NINT/28 May 2008

From 2008.igem.org

(Difference between revisions)
 
(3 intermediate revisions not shown)
Line 1: Line 1:
-
lab work (SD): Transformed XL1-B cells with the plasmids created 27/05/08.  K102002 had 170 colonies.  K102004 had 629 colonies.  K102000 negative control had 5 colonies.
+
[[Team:Alberta_NINT/Notebook | < Back to notebook]]
-
lab work (JD): Carried out mini-preps on O/Ns, digested R0011 with SpeI+ PstI, ran gel electrophoresis and cut out desired band.
+
[[Alberta_NINT/27_May_2008 | < Previous entry]] | [[Alberta_NINT/29_May_2008 | Next entry >]]
 +
 
 +
 
 +
lab work (SD):
 +
 
 +
        Transformed XL1-B cells with the plasmids created 27/05/08. 
 +
        K102002 had 170 colonies.  K102004 had 629 colonies.  K102000 negative control had 5 colonies.
 +
 
 +
lab work (JD):  
 +
 
 +
          Carried out mini-preps on O/Ns
 +
          Digested R0011 with SpeI+ PstI
 +
          Ran gel electrophoresis and cut out desired band.
 +
 
 +
lab work (WM):
 +
          Ligated I0500/(SpeI + PstI) with TA0In/(XbaI + PstI)
 +
          Transformed 2 ul into 100 ul XL1-B. 
 +
          Plated 150 ul on LB + kan35

Latest revision as of 21:38, 11 October 2008

< Back to notebook

< Previous entry | Next entry >


lab work (SD): 

        Transformed XL1-B cells with the plasmids created 27/05/08.  
        K102002 had 170 colonies.  K102004 had 629 colonies.  K102000 negative control had 5 colonies.

lab work (JD): 

         Carried out mini-preps on O/Ns
         Digested R0011 with SpeI+ PstI
         Ran gel electrophoresis and cut out desired band.

lab work (WM): 

         Ligated I0500/(SpeI + PstI) with TA0In/(XbaI + PstI) 
         Transformed 2 ul into 100 ul XL1-B.  
         Plated 150 ul on LB + kan35
Retrieved from "http://2008.igem.org/Alberta_NINT/28_May_2008"