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Alberta NINT/16 May 2008
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(New page: lab work (JD): PCR of T/A Sequence, Digested T/A Sequence with Pst, Xba, Ran gel electrophoresis.) |
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| - | lab work (JD): PCR of T/A | + | [[Team:Alberta_NINT/Notebook | < Back to notebook]] |
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| + | lab work (SD, JD): | ||
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| + | PCR of T/A I oligonucleotide using Term + and Term - primers | ||
| + | DNA digest of T/A I with XbaI + PstI to isolate the terminator and establish sticky ends for | ||
| + | insertion into plasmid. | ||
| + | Ran on 2% agarose gel. | ||
| + | [[Image:UVP00296annotated.jpg]] | ||
Latest revision as of 21:40, 11 October 2008
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lab work (SD, JD):
PCR of T/A I oligonucleotide using Term + and Term - primers
DNA digest of T/A I with XbaI + PstI to isolate the terminator and establish sticky ends for
insertion into plasmid.
Ran on 2% agarose gel.
