Alberta NINT/4 June 2008

From 2008.igem.org

(Difference between revisions)

Latest revision as of 22:17, 11 October 2008

lab work (SD):

                   Transformed XL1-B cells with plasmids K102008, K102009 and negative controls.

lab work (JD):

                     From sequencing results K102001.1 becomes the K102001.  
                     Digested K102001 with SpeI and PstI.  
                     Ran gel electrophoresis and cut out desired band ~2500bp

lab work (WM):

                 Miniprep 4 O/Ns of K102011 transformants (2a, 2b, 3a, 3b)

@@ Line 1: / Line 1: @@
-lab work (SD): Transformed XL1-B cells with plasmids K102008, K102009 and negative controls.
+[[Team:Alberta_NINT/Notebook | < Back to notebook]]
-lab work (JD): From sequencing results K102001.1 becomes the K102001.  Digested K102001 with SpeI and PstI.  Ran gel electrophoresis and cut out desired bankd ~2500bp
+[[Alberta_NINT/3_June_2008 | < Previous entry]] | [[Alberta_NINT/5_June_2008 | Next entry >]]
+lab work (SD):
+                    Transformed XL1-B cells with plasmids K102008, K102009 and negative controls.
+lab work (JD):
+                      From sequencing results K102001.1 becomes the K102001.
+                      Digested K102001 with SpeI and PstI.
+                      Ran gel electrophoresis and cut out desired band ~2500bp
+                      [[Image:slide2.jpg]]
+lab work (WM):
+                  Miniprep 4 O/Ns of K102011 transformants (2a, 2b, 3a, 3b)