Alberta NINT/3 June 2008
From 2008.igem.org
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lab work (SD):
Ran K102002/B+N and K102004/B+N on 2% agarose gel to purify. Used QIAquick nucleotide removal standard protocol to purify T/A 0. Ligated T/A 0 into K102002/B+N and K102004/B+N.
lab work (WM):
#colonies K102011: 48 negative ctl: 0 Picked 4 colonies for O/Ns