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Alberta NINT/4 June 2008
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[[Team:Alberta_NINT/Notebook | < Back to notebook]] | [[Team:Alberta_NINT/Notebook | < Back to notebook]] | ||
| - | [[Alberta_NINT/3_June_2008 | < Previous entry]] | + | [[Alberta_NINT/3_June_2008 | < Previous entry]] | [[Alberta_NINT/5_June_2008 | Next entry >]] |
lab work (SD): | lab work (SD): | ||
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Digested K102001 with SpeI and PstI. | Digested K102001 with SpeI and PstI. | ||
Ran gel electrophoresis and cut out desired band ~2500bp | Ran gel electrophoresis and cut out desired band ~2500bp | ||
| + | [[Image:slide2.jpg]] | ||
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| + | lab work (WM): | ||
| + | Miniprep 4 O/Ns of K102011 transformants (2a, 2b, 3a, 3b) | ||
Latest revision as of 22:17, 11 October 2008
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lab work (SD):
Transformed XL1-B cells with plasmids K102008, K102009 and negative controls.
lab work (JD):
From sequencing results K102001.1 becomes the K102001.
Digested K102001 with SpeI and PstI.
Ran gel electrophoresis and cut out desired band ~2500bp
lab work (WM):
Miniprep 4 O/Ns of K102011 transformants (2a, 2b, 3a, 3b)
