Alberta NINT/17 June 2008

From 2008.igem.org

(Difference between revisions)
(New page: lab work (SD): Transformed XL1-B cells with: K102000 + I732018.1 K102000 + I732018.2 K102000 (negative control))
 
(3 intermediate revisions not shown)
Line 1: Line 1:
 +
[[Team:Alberta_NINT/Notebook | < Back to notebook]]
 +
 +
[[Alberta_NINT/16_June_2008 | < Previous entry]] | [[Alberta_NINT/18_June_2008 | Next entry >]]
 +
 +
lab work (SD):
lab work (SD):
             Transformed XL1-B cells with:
             Transformed XL1-B cells with:
Line 4: Line 9:
                   K102000 + I732018.2
                   K102000 + I732018.2
                   K102000 (negative control)
                   K102000 (negative control)
 +
 +
lab work (JD):
 +
              Re-created K102003 with new LacZ parts I732018.1 and I732018.2
 +
              Ligated K102001 with LacZ Parts
 +
              Transformed and plated XL1-B cells with K102003(1) (containing LacZ part I732018.1) and K102003
 +
              (2) (containing LacZ part I732018.2)
 +
              Our plates of K102007 from yesterday with 1/10 1/100 and 1/1000 dilutions didn't seem to make any
 +
              difference so we are going to recut TA0
 +
              Restriction digest of TA0 with BamHI and NcoI
 +
              Nucleotide removal of TA0/B+N
 +
              Created glycerol stock of K102005
 +
              Made fresh O/N of K102005 to be used tomorrow

Latest revision as of 22:51, 11 October 2008

< Back to notebook

< Previous entry | Next entry >


lab work (SD): 

            Transformed XL1-B cells with:
                 K102000 + I732018.1
                 K102000 + I732018.2
                 K102000 (negative control)

lab work (JD): 

              Re-created K102003 with new LacZ parts I732018.1 and I732018.2
              Ligated K102001 with LacZ Parts
              Transformed and plated XL1-B cells with K102003(1) (containing LacZ part I732018.1) and K102003
              (2) (containing LacZ part I732018.2)
              Our plates of K102007 from yesterday with 1/10 1/100 and 1/1000 dilutions didn't seem to make any 
              difference so we are going to recut TA0
              Restriction digest of TA0 with BamHI and NcoI
              Nucleotide removal of TA0/B+N
              Created glycerol stock of K102005
              Made fresh O/N of K102005 to be used tomorrow
Retrieved from "http://2008.igem.org/Alberta_NINT/17_June_2008"