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Alberta NINT/25 June 2008
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[[Team:Alberta_NINT/Notebook | < Back to notebook]] | [[Team:Alberta_NINT/Notebook | < Back to notebook]] | ||
| - | [[Alberta_NINT/24_June_2008 | < Previous entry]] | + | [[Alberta_NINT/24_June_2008 | < Previous entry]] | [[Alberta_NINT/26_June_2008 | Next entry>]] |
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| + | lab work (SD): | ||
| + | Isolated DNA (K102008) from 24/06/08 overnights using QIAprep spin miniprep protocol. | ||
| + | Set up K102008 for sequencing. | ||
| + | Digested K102002 and K102008 with EcoRI + PstI, ran on 2% agarose gel at 110V for 50 min. | ||
| + | Excised large fragments from the gel. | ||
| + | Set up 8 overnights from the 14 colonies found on the K102008 plate from 24/06/08. | ||
| + | [[Image:NINT_SD_p52.jpg|750 px]] | ||
lab work (JD): | lab work (JD): | ||
| - | Decided to try AGAIN to recreate K102007 but using older versions of K102005/B+N that seemed to be cut better when looking at gels and ligating them to the newly | + | Decided to try AGAIN to recreate K102007 but using older versions of K102005/B+N that seemed to be |
| + | cut better when looking at gels and ligating them to the newly annealed TA0/B+N (a combination I | ||
| + | still haven't tried) | ||
Created K102007(1): K102005/B+N from June 18, and TA0/B+N newly annealed | Created K102007(1): K102005/B+N from June 18, and TA0/B+N newly annealed | ||
Created K102007(2): K102005/B+N from June 12 and TA0/B+N newly annealed | Created K102007(2): K102005/B+N from June 12 and TA0/B+N newly annealed | ||
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Transformed XL1-B cells with the ligations and negative controls | Transformed XL1-B cells with the ligations and negative controls | ||
Plated cells | Plated cells | ||
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Latest revision as of 22:54, 11 October 2008
< Previous entry | Next entry>
lab work (SD):
Isolated DNA (K102008) from 24/06/08 overnights using QIAprep spin miniprep protocol.
Set up K102008 for sequencing.
Digested K102002 and K102008 with EcoRI + PstI, ran on 2% agarose gel at 110V for 50 min.
Excised large fragments from the gel.
Set up 8 overnights from the 14 colonies found on the K102008 plate from 24/06/08.
lab work (JD):
Decided to try AGAIN to recreate K102007 but using older versions of K102005/B+N that seemed to be
cut better when looking at gels and ligating them to the newly annealed TA0/B+N (a combination I
still haven't tried)
Created K102007(1): K102005/B+N from June 18, and TA0/B+N newly annealed
Created K102007(2): K102005/B+N from June 12 and TA0/B+N newly annealed
Created K102006: K102003/B+N and TA0/B+N newly annealed
Transformed XL1-B cells with the ligations and negative controls
Plated cells
