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Team:Warsaw/Calendar-Main/24 July 2008
From 2008.igem.org
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| - | <h3>Cloning of protein Z DNA to pET15b-OmpA-omega in place of OmpA</h3><h4>Paweł</h4> | + | <h3>Cloning of protein Z DNA to <a href=https://2008.igem.org/Wiki/Team:Warsaw/vectors/pET15b%2BOmpA-omega>pET15b-OmpA-omega</a> in place of OmpA</h3><h4>Paweł</h4> |
<ol><li>Results of ligation: 6 colonies grown.</li> | <ol><li>Results of ligation: 6 colonies grown.</li> | ||
<li>Each colony cultured overnight in LB + ampicillin.</li></ol> | <li>Each colony cultured overnight in LB + ampicillin.</li></ol> | ||
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<h3>Cloning of protein A DNA to OmpA constructs</h3><h4>Michał K.</h4> | <h3>Cloning of protein A DNA to OmpA constructs</h3><h4>Michał K.</h4> | ||
<ol> | <ol> | ||
| - | <li>Colony PCR on colonies from plates with transformations pACYC177+OmpA_A_omega. Primers used: | + | <li>Colony PCR on colonies from plates with transformations <a href=https://2008.igem.org/Wiki/Team:Warsaw/vectors/pACYC177%2BompA-A-omega>pACYC177+OmpA_A_omega</a>. Primers used: |
<a href="https://2008.igem.org/Wiki/Team:Warsaw/primers#AL+SacI">AL+SacI</a> and <a href="https://2008.igem.org/Wiki/Team:Warsaw/primers#AP+NotI">AP+NotI</a>.</li><li> Gel electrophoresis.</li> | <a href="https://2008.igem.org/Wiki/Team:Warsaw/primers#AL+SacI">AL+SacI</a> and <a href="https://2008.igem.org/Wiki/Team:Warsaw/primers#AP+NotI">AP+NotI</a>.</li><li> Gel electrophoresis.</li> | ||
Revision as of 21:58, 14 October 2008
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Cloning of protein Z DNA to pET15b-OmpA-omega in place of OmpAPaweł
Cloning of protein A DNA to OmpA constructsMichał K.
AntoniPreparation of chemocompetent bacteria E. coli TOP10.
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