Team:Hawaii/Notebook/2008-10-14
From 2008.igem.org
(Difference between revisions)
(New page: {{Team:Hawaii/Header}} = Things we did today = == Wetlab work == ===Construction of secretion device (cont.)=== :<strong> Grace</strong> [[Image:101408REdigest.png|right|thumb|300px|EtBr ...) |
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::* nrsg6 (from 10/11) + BBpRL1383a-1 | ::* nrsg6 (from 10/11) + BBpRL1383a-1 | ||
::* J33207 + pRL1383a | ::* J33207 + pRL1383a | ||
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+ | ==Drylab Work== | ||
+ | ===Sequencing analysis=== | ||
+ | :<strong>Grace</strong> | ||
+ | :* prpgt, nrsg, sgt, and pgt sequences all returned inserts of ''E. coli'' genomic DNA instead of desired construct | ||
= Discussion = | = Discussion = |
Revision as of 18:22, 15 October 2008
Projects | Events | Resources | ||
---|---|---|---|---|
Sponsors | Experiments | Milestones | Protocols | |
Notebook (t) | Meetings (t) |
Things we did today
Wetlab work
Construction of secretion device (cont.)
- Grace
- Ran RE digests on gel
- Extracted rgt1 and prpgt5 from gel
- Ligated:
- prpgt5 + BBpRL1383a-1
- nir + rgt1 + BBpRL1383a-1
- plac + rgt1 + BBpRL1383a-1
- nrsg6 (from 10/11) + BBpRL1383a-1
- J33207 + pRL1383a
Drylab Work
Sequencing analysis
- Grace
- prpgt, nrsg, sgt, and pgt sequences all returned inserts of E. coli genomic DNA instead of desired construct
Discussion
Quote of the Day
History is the only laboratory we have in which to test the consequences of thought. - Étienne Gilson
[http://manoa.hawaii.edu/ ][http://manoa.hawaii.edu/ovcrge/ ][http://www.ctahr.hawaii.edu ]