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Team:Hawaii/Notebook/2008-06- 5
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{{Team:Hawaii/Header}} | {{Team:Hawaii/Header}} | ||
| - | + | = What we did today = | |
| - | ''' | + | == Wetlab work == |
| - | + | ===Made PCR gel=== | |
| - | + | :'''Norman''' | |
:* 12g Agarose | :* 12g Agarose | ||
:* 12 ml 50X TAE | :* 12 ml 50X TAE | ||
:* 588 ml nanopure water | :* 588 ml nanopure water | ||
:* 6 μl EtBr | :* 6 μl EtBr | ||
| - | + | ||
| + | ===Transformed DH5α with PRL1383a === | ||
| + | :'''Grace & Krystle''' | ||
| + | :* Used same protocol as competency test on 6/3/08 | ||
:* Shaking at 235 rpm | :* Shaking at 235 rpm | ||
:* Plated on LB+sp plate | :* Plated on LB+sp plate | ||
:* E. coli from batch 2 (2-14) (OD600=0.3) | :* E. coli from batch 2 (2-14) (OD600=0.3) | ||
| - | + | = Discussion = | |
* GK and KS will read up on PCC6803 transformation tonight; transformation will be conducted tomorrow morning. | * GK and KS will read up on PCC6803 transformation tonight; transformation will be conducted tomorrow morning. | ||
* Tomorrow afternoon, we will practice extracting BioBrick plasmids off filter paper. | * Tomorrow afternoon, we will practice extracting BioBrick plasmids off filter paper. | ||
* Also need to make TE tomorrow. | * Also need to make TE tomorrow. | ||
| - | + | = Quote of the day = | |
| - | "So Grace, what promoter are going use?"<br> | + | <blockquote>"So Grace, what promoter are you going use?"<br> |
| - | "Um... my name is Krystle." | + | :::"Um... my name is Krystle."</blockquote> |
| - | (an hour later, via Skype) | + | <html><align=center>(an hour later, via Skype)</align></html> |
| - | "Okay, see you all next week. Bye Grace... er... Margaret." | + | <blockquote>"Okay, see you all next week. Bye Grace... er... Margaret."<blockquote> |
{{Team:Hawaii/Footer}} | {{Team:Hawaii/Footer}} | ||
Latest revision as of 03:27, 12 June 2008
| Projects | Events | Resources | ||
|---|---|---|---|---|
| Sponsors | Experiments | Milestones | Protocols | |
| Notebook (t) | Meetings (t) |
Contents |
What we did today
Wetlab work
Made PCR gel
- Norman
- 12g Agarose
- 12 ml 50X TAE
- 588 ml nanopure water
- 6 μl EtBr
Transformed DH5α with PRL1383a
- Grace & Krystle
- Used same protocol as competency test on 6/3/08
- Shaking at 235 rpm
- Plated on LB+sp plate
- E. coli from batch 2 (2-14) (OD600=0.3)
Discussion
- GK and KS will read up on PCC6803 transformation tonight; transformation will be conducted tomorrow morning.
- Tomorrow afternoon, we will practice extracting BioBrick plasmids off filter paper.
- Also need to make TE tomorrow.
Quote of the day
"So Grace, what promoter are you going use?"
- "Um... my name is Krystle."
"Okay, see you all next week. Bye Grace... er... Margaret."[http://manoa.hawaii.edu/][http://manoa.hawaii.edu/ovcrge/
][http://www.ctahr.hawaii.edu
]
