Team:Warsaw/Calendar-Main/24 May 2008

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<h3> Results of rifampicin test</h3>
<h3> Results of rifampicin test</h3>
<h4>Piotr</h4>
<h4>Piotr</h4>
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><h3>Preparation of <a href=https://2008.igem.org/Wiki/Team:Warsaw/vectors/pMPM-T5%2BAID%2BT7>pMPMT5-AID+T7</a> and <a href=https://2008.igem.org/Wiki/Team:Warsaw/vectors/pMPM-T5%2BAIDT7>pMPMT5+AID-T7</a></h3>
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<h3>Preparation of <a href=https://2008.igem.org/Wiki/Team:Warsaw/vectors/pMPM-T5%2BAID%2BT7>pMPMT5-AID+T7</a> and <a href=https://2008.igem.org/Wiki/Team:Warsaw/vectors/pMPM-T5%2BAIDT7>pMPMT5+AID-T7</a></h3>
<h4>Michał K.</h4>
<h4>Michał K.</h4>

Revision as of 11:51, 19 October 2008

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Results of rifampicin test

Piotr

StrainInducer / repressorNumber of colonies on rifampicin
Top10none19
Top10 pMPM-AIDnone10
Top10 pMPM-AID0.1% Arabinose154
Top10 pTRC-AIDnone11
Top10 pTRC-AID0.5 mM IPTG59

Conclusion: AID seems to work!

Preparation of pMPMT5-AID+T7 and pMPMT5+AID-T7

Michał K.

  1. Isolation of hypothetical pMPM-T5 with transcriptional fusion from cultures.
  2. Isolation of hypothetical pMPM-T5 with translational fusion from cultures.
  3. Control digest of pMPM-T5 with transcriptional fusionwith EcoRI (EcoRI buffer).
  4. Control digest of pMPM-T5 with translational fusion with BamHI (BamHI buffer).
  5. Gel electrophoresis (still no proper colonies found).

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