Team:Warsaw/Calendar-Main/11 June 2008

(Difference between revisions)

Revision as of 13:20, 19 October 2008


	Attribution analyzer / Interactive list of topics

Transformation of E. coli TOP10 carrying pZC320 with:
- pMPMT5-omega,
- pMPMT5-AID,
- pMPMT5-AIDT7,
- pMPMT5-AID+T7.
Plating transformants on 'TAXI' (LB + Tetracycline + IPTG + Ampicillin 30 μg/mL + X-Gal).
Plating transformants on liquid LB + Tetracycline + Ampicillin 30 μg/mL

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-<h3> Blue/white and rifampicin test </h3><h4>Michał L., Ewa, Marcin</h4>
+<h3> Blue/white and rifampicin test </h3><h4>Michał L., Ewa, Piotr</h4>
 <ol>
 <li><a href="https://2008.igem.org/wiki/index.php?title=Wiki/Team:Warsaw/protocols#chemotransform">Transformation</a> of <i>E. coli</i> <a href="https://2008.igem.org/Wiki/Team:Warsaw/igem_notebook.htm#top10">TOP10</a> carrying <a href="https://2008.igem.org/Wiki/Team:Warsaw/vectors/pZC320">pZC320</a> with:
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 <li><a href=https://2008.igem.org/Wiki/Team:Warsaw/vectors/pMPM-T5%2BAID%2BT7>pMPMT5-AID+T7</a>.</li></ul>
 <li>Plating transformants on 'TAXI' (LB + Tetracycline + IPTG + Ampicillin 30 μg/mL + X-Gal).</li>
+<li>Plating transformants on liquid LB + Tetracycline + Ampicillin 30 μg/mL</li>
 </html>