Team:Valencia/Notebook/September
From 2008.igem.org
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+ | ===September 2nd === | ||
+ | -We carried out the following experiment: <br> | ||
+ | Four strains in LCCs with [[Team:Valencia/Notebook/Protocols#SP liquid medium | SP medium]]. <br> | ||
+ | 1 % Galactose induction at the beginning. Initial O.D. 0,7 <br> | ||
+ | 275 rpm. <br> | ||
+ | === September 3rd === | ||
+ | -We characterized LCC temperature evolution with water. <br> | ||
+ | |||
+ | ===September 5th === | ||
+ | -We characterized LCC temperature evolution with [[Team:Valencia/Notebook/Protocols#SP liquid medium | SP medium]] and SP medium with bleach (to avoid contamination). | ||
+ | |||
+ | === September 8th === | ||
+ | - We observed the culture after the experiment under the microcope. There was no contamination but we did observe some elongated microorganisms, same size as ''S. cerevisiae''. <br> | ||
+ | |||
+ | - We discovered that our plaque with the four strains for the following experiment had suffered cross contamination. <br> | ||
+ | |||
+ | === September 9th === | ||
+ | -We carried out the following experiment: <br> | ||
+ | Four strains in LCCs with [[Team:Valencia/Notebook/Protocols#SP liquid medium | SP medium]]. <br> | ||
+ | 1 % Galactose induction at the beginning. Initial O.D. 0,6 <br> | ||
+ | 270 rpm. <br> | ||
+ | Initial temperature 31 ºC. Too high. <br> | ||
+ | |||
+ | === September 11th === | ||
+ | -We repeated the same experiment: <br> | ||
+ | Four strains in LCCs with [[Team:Valencia/Notebook/Protocols#SP liquid medium | SP medium]]. <br> | ||
+ | 1 % Galactose induction at the beginning. Initial O.D. 0,6 <br> | ||
+ | 270 rpm. <br> | ||
+ | Initial temperature 31 ºC. Too high again. <br> | ||
+ | |||
+ | === September 15th === | ||
+ | -We repeated the same experiment: <br> | ||
+ | Four strains in LCCs with [[Team:Valencia/Notebook/Protocols#SP liquid medium | SP medium]]. <br> | ||
+ | 1 % Galactose induction at the beginning. Initial O.D. 0,6 <br> | ||
+ | 270 rpm. <br> | ||
+ | Initial temperature 28 ºC. Perfect! <br> | ||
+ | |||
+ | === September 20th === | ||
+ | -Trying to reproduce the previous experiment... | ||
+ | Four strains in LCCs with [[Team:Valencia/Notebook/Protocols#SP liquid medium | SP medium]]. <br> | ||
+ | 1 % Galactose induction at the beginning. Initial O.D. 0,6 <br> | ||
+ | 270 rpm. <br> | ||
+ | Initial temperature 28 ºC. But room temperature too high <br> | ||
Revision as of 15:30, 19 October 2008
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September 2nd
-We carried out the following experiment:
Four strains in LCCs with SP medium.
1 % Galactose induction at the beginning. Initial O.D. 0,7
275 rpm.
September 3rd
-We characterized LCC temperature evolution with water.
September 5th
-We characterized LCC temperature evolution with SP medium and SP medium with bleach (to avoid contamination).
September 8th
- We observed the culture after the experiment under the microcope. There was no contamination but we did observe some elongated microorganisms, same size as S. cerevisiae.
- We discovered that our plaque with the four strains for the following experiment had suffered cross contamination.
September 9th
-We carried out the following experiment:
Four strains in LCCs with SP medium.
1 % Galactose induction at the beginning. Initial O.D. 0,6
270 rpm.
Initial temperature 31 ºC. Too high.
September 11th
-We repeated the same experiment:
Four strains in LCCs with SP medium.
1 % Galactose induction at the beginning. Initial O.D. 0,6
270 rpm.
Initial temperature 31 ºC. Too high again.
September 15th
-We repeated the same experiment:
Four strains in LCCs with SP medium.
1 % Galactose induction at the beginning. Initial O.D. 0,6
270 rpm.
Initial temperature 28 ºC. Perfect!
September 20th
-Trying to reproduce the previous experiment...
Four strains in LCCs with SP medium.
1 % Galactose induction at the beginning. Initial O.D. 0,6
270 rpm.
Initial temperature 28 ºC. But room temperature too high