Team:Valencia/Notebook/September
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+ | <html><link rel="stylesheet" href="https://2008.igem.org/wiki/index.php?title=User:Joadelas/valencia.css&action=raw&ctype=text/css" type="text/css"></html> | ||
+ | {{Valencia/Menu}} | ||
+ | <div class="valenciaMain"> | ||
<div align="center"> | <div align="center"> | ||
{| | {| | ||
- | | valign="top" | | + | || || valign="top" | |
+ | <!-- {{{Año}}}/04 (Abril) --> | ||
+ | {| | ||
+ | | colspan="7" style="background:#FFD062;" | <Center>[[Team:Valencia/Notebook/April|<font color="#047DB5">April</font>]]</Center> | ||
+ | |- bgcolor="#FFE099" | ||
+ | | Mo || Tu || We || Th || Fr || Sa || <Font Color="#FF0000">Su</Font> | ||
+ | |- | ||
+ | | <Font Color="#c0c0c0">31</Font> || 1 || 2 || 3 || 4 || 5 || 6 | ||
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+ | | 28 || 29 || 30 || <Font Color="#c0c0c0">1</Font> || <Font Color="#c0c0c0">2</Font> || <Font Color="#c0c0c0">3</Font> || <Font Color="#c0c0c0">4</Font> | ||
+ | |} | ||
+ | || || valign="top" | | ||
+ | <!-- {{{Año}}}/05 (Mayo) --> | ||
+ | {| | ||
+ | | colspan="7" style="background:#FFD062;" | <Center>[[Team:Valencia/Notebook/May|<font color="#047DB5">May</font>]]</Center> | ||
+ | |- bgcolor="#FFE099" | ||
+ | | Mo || Tu || We || Th || Fr || Sa || <Font Color="#FF0000">Su</Font> | ||
+ | |- | ||
+ | | <Font Color="#c0c0c0">28</Font> || <Font Color="#c0c0c0">29</Font> || <Font Color="#c0c0c0">30</Font> || 1 || 2 || 3 || 4 | ||
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+ | | 12 || 13 || 14 || 15 || 16 || 17 || 18 | ||
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+ | | 19 || 20 || 21 || 22 || 23 || 24 || 25 | ||
+ | |- | ||
+ | | 26 || 27 || 28 || 29 || 30 || 31 || <Font Color="#c0c0c0">1</Font> | ||
+ | |} | ||
+ | || || valign="top" | | ||
+ | <!-- {{{Año}}}/06 (Junio) --> | ||
+ | {| | ||
+ | | colspan="7" style="background:#FFD062;" | <Center>[[Team:Valencia/Notebook/June|<font color="#047DB5">June</font>]]</Center> | ||
+ | |- bgcolor="#FFE099" | ||
+ | | Mo || Tu || We || Th || Fr || Sa || <Font Color="#FF0000">Su</Font> | ||
+ | |- | ||
+ | | <Font Color="#c0c0c0">26</Font> || <Font Color="#c0c0c0">27</Font> || <Font Color="#c0c0c0">28</Font> || <Font Color="#c0c0c0">29</Font> || <Font Color="#c0c0c0">30</Font> || <Font Color="#c0c0c0">31</Font> || 1 | ||
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+ | | 2 || 3 || 4 || 5 || 6 || 7 || 8 | ||
+ | |- | ||
+ | | 9 || 10 || 11 || 12 || 13 || 14 || 15 | ||
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+ | | 16 || 17 || 18 || 19 || 20 || 21 || 22 | ||
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+ | | 30 || <Font Color="#c0c0c0">1</Font> || <Font Color="#c0c0c0">2</Font> || <Font Color="#c0c0c0">3</Font> || <Font Color="#c0c0c0">4</Font> || <Font Color="#c0c0c0">5</Font> || <Font Color="#c0c0c0">6</Font> | ||
+ | |} | ||
+ | || || valign="top" | | ||
<!-- {{{Año}}}/07 (Julio) --> | <!-- {{{Año}}}/07 (Julio) --> | ||
- | {| | + | {| |
- | | colspan="7" style="background:#FFD062;" | <Center>[[Team:Valencia/Notebook/July|July]]</Center> | + | | colspan="7" style="background:#FFD062;" | <Center>[[Team:Valencia/Notebook/July|<font color="#047DB5">July</font>]]</Center> |
|- bgcolor="#FFE099" | |- bgcolor="#FFE099" | ||
| Mo || Tu || We || Th || Fr || Sa || <Font Color="#FF0000">Su</Font> | | Mo || Tu || We || Th || Fr || Sa || <Font Color="#FF0000">Su</Font> | ||
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| 28|| 29 || 30 || 31 || <Font Color="#c0c0c0"> 1</Font> || <Font Color="#c0c0c0"> 2</Font> || <Font Color="#c0c0c0"> 3</Font> | | 28|| 29 || 30 || 31 || <Font Color="#c0c0c0"> 1</Font> || <Font Color="#c0c0c0"> 2</Font> || <Font Color="#c0c0c0"> 3</Font> | ||
|} | |} | ||
+ | |} | ||
+ | {| | ||
|| || valign="top" | | || || valign="top" | | ||
<!-- {{{Año}}}/08 (Agosto) --> | <!-- {{{Año}}}/08 (Agosto) --> | ||
- | {| | + | {| |
- | | colspan="7" style="background:#FFD062;" | <Center>[[Team:Valencia/Notebook/August|August]]</Center> | + | | colspan="7" style="background:#FFD062;" | <Center>[[Team:Valencia/Notebook/August|<font color="#047DB5">August</font>]]</Center> |
|- bgcolor="#FFE099" | |- bgcolor="#FFE099" | ||
| Mo || Tu || We || Th || Fr || Sa || <Font Color="#FF0000">Su</Font> | | Mo || Tu || We || Th || Fr || Sa || <Font Color="#FF0000">Su</Font> | ||
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|| || valign="top" | | || || valign="top" | | ||
<!-- {{{Año}}}/09 (Septiembre) --> | <!-- {{{Año}}}/09 (Septiembre) --> | ||
- | {| | + | {| |
- | | colspan="7" style="background:#FFB428;" | <Center>[[Team:Valencia/Notebook/September|September]]</Center> | + | | colspan="7" style="background:#FFB428;" | <Center>[[Team:Valencia/Notebook/September|<font color="#047DB5">September</font>]]</Center> |
|- bgcolor="#FFE099" | |- bgcolor="#FFE099" | ||
| Mo || Tu || We || Th || Fr || Sa || <Font Color="#FF0000">Su</Font> | | Mo || Tu || We || Th || Fr || Sa || <Font Color="#FF0000">Su</Font> | ||
|- | |- | ||
- | | 1 || 2 || 3 || 4 || 5 || 6|| 7 | + | | 1 || [[Team:Valencia/Notebook/September #September 2nd|2]] || [[Team:Valencia/Notebook/September #September 3rd|3]] || 4 || [[Team:Valencia/Notebook/September #September 5th|5]] || 6|| 7 |
|- | |- | ||
- | | 8 || 9 || 10 || 11 || 12 || 13 || 14 | + | | [[Team:Valencia/Notebook/September #September 8th|8]] || [[Team:Valencia/Notebook/September #September 9th|9]] || 10 || [[Team:Valencia/Notebook/September #September 11th|11]] || 12 || 13 || 14 |
|- | |- | ||
- | | 15 || 16 || 17 || 18 || 19 || 20|| 21 | + | | [[Team:Valencia/Notebook/September #September 15th|15]] || 16 || 17 || 18 || 19 || [[Team:Valencia/Notebook/September #September 20th|20]] || 21 |
|- | |- | ||
- | | 22 || 23 || 24 || 25 || 26 || 27 || 28 | + | | 22 || 23 || [[Team:Valencia/Notebook/September #September 24th|24]] || 25 || [[Team:Valencia/Notebook/September #September 26th|26]] || [[Team:Valencia/Notebook/September #September 27th|27]] || 28 |
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<!-- {{{Año}}}/10 (Octubre) --> | <!-- {{{Año}}}/10 (Octubre) --> | ||
- | {| | + | {| |
- | | colspan="7" style="background:#FFD062;" | <Center>[[Team:Valencia/Notebook/October|October]]</Center> | + | | colspan="7" style="background:#FFD062;" | <Center>[[Team:Valencia/Notebook/October|<font color="#047DB5">October</font>]]</Center> |
|- bgcolor="#FFE099" | |- bgcolor="#FFE099" | ||
| Mo || Tu || We || Th || Fr || Sa || <Font Color="#FF0000">Su</Font> | | Mo || Tu || We || Th || Fr || Sa || <Font Color="#FF0000">Su</Font> | ||
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<!-- {{{Año}}}/11 (Noviembre) --> | <!-- {{{Año}}}/11 (Noviembre) --> | ||
- | {| | + | {| |
- | | colspan="7" style="background:#FFD062;" | <Center>[[Team:Valencia/Notebook/November|November]]</Center> | + | | colspan="7" style="background:#FFD062;" | <Center>[[Team:Valencia/Notebook/November|<font color="#047DB5">November</font>]]</Center> |
|- bgcolor="#FFE099" | |- bgcolor="#FFE099" | ||
| Mo || Tu || We || Th || Fr || Sa || <Font Color="#FF0000">Su</Font> | | Mo || Tu || We || Th || Fr || Sa || <Font Color="#FF0000">Su</Font> | ||
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|} | |} | ||
|} | |} | ||
+ | </div> | ||
+ | |||
+ | |||
+ | <div style=" width:94%; margin: 0 auto;"> | ||
+ | <!-- ESCRIBIR AQUI --> | ||
+ | |||
+ | ===September 2nd === | ||
+ | -We carried out the following experiment: <br> | ||
+ | Four strains in LCCs with [[Team:Valencia/Notebook/Protocols#SP liquid medium | SP medium]]. <br> | ||
+ | 1 % Galactose induction at the beginning. Initial O.D. 0,7 <br> | ||
+ | 275 rpm. <br> | ||
+ | |||
+ | -Team meeting: Checking project progress. | ||
+ | |||
+ | === September 3rd === | ||
+ | -We characterized LCC temperature evolution with water. <br> | ||
+ | |||
+ | ===September 5th === | ||
+ | -We characterized LCC temperature evolution with [[Team:Valencia/Notebook/Protocols#SP liquid medium | SP medium]] and SP medium with bleach (to avoid contamination). | ||
+ | |||
+ | === September 8th === | ||
+ | - We observed the culture after the experiment under the microcope. There was no contamination but we did observe some elongated microorganisms, same size as ''S. cerevisiae''. <br> | ||
+ | |||
+ | - We discovered that our plaque with the four strains for the following experiment had suffered cross contamination. <br> | ||
+ | |||
+ | === September 9th === | ||
+ | -We carried out the following experiment: <br> | ||
+ | Four strains in LCCs with [[Team:Valencia/Notebook/Protocols#SP liquid medium | SP medium]]. <br> | ||
+ | 1 % Galactose induction at the beginning. Initial O.D. 0,6 <br> | ||
+ | 270 rpm. <br> | ||
+ | Initial temperature 31 ºC. Too high. <br> | ||
+ | |||
+ | === September 11th === | ||
+ | -We repeated the same experiment: <br> | ||
+ | Four strains in LCCs with [[Team:Valencia/Notebook/Protocols#SP liquid medium | SP medium]]. <br> | ||
+ | 1 % Galactose induction at the beginning. Initial O.D. 0,6 <br> | ||
+ | 270 rpm. <br> | ||
+ | Initial temperature 31 ºC. Too high again. <br> | ||
+ | |||
+ | === September 15th === | ||
+ | -We repeated the same experiment: <br> | ||
+ | Four strains in LCCs with [[Team:Valencia/Notebook/Protocols#SP liquid medium | SP medium]]. <br> | ||
+ | 1 % Galactose induction at the beginning. Initial O.D. 0,6 <br> | ||
+ | 270 rpm. <br> | ||
+ | Initial temperature 28 ºC. Perfect! <br> | ||
+ | |||
+ | === September 20th === | ||
+ | -Trying to reproduce the previous experiment... | ||
+ | Four strains in LCCs with [[Team:Valencia/Notebook/Protocols#SP liquid medium | SP medium]]. <br> | ||
+ | 1 % Galactose induction at the beginning. Initial O.D. 0,6 <br> | ||
+ | 270 rpm. <br> | ||
+ | Initial temperature 28 ºC. But room temperature too high <br> | ||
+ | |||
+ | === September 24th === | ||
+ | - We repeated the same experiment: <br> | ||
+ | Four strains in LCCs with [[Team:Valencia/Notebook/Protocols#SP liquid medium | SP medium]]. <br> | ||
+ | 1 % Galactose induction at the beginning. Initial O.D. 0,6 <br> | ||
+ | 270 rpm. <br> | ||
+ | Initial temperature 28 ºC.<br> | ||
+ | |||
+ | === September 26th === | ||
+ | - Team meeting: Checking project progress and inmediate future actions. | ||
+ | |||
+ | === September 27th === | ||
+ | - We tryied and increased initial O.D. <br> | ||
+ | Four strains in LCCs with [[Team:Valencia/Notebook/Protocols#SP liquid medium | SP medium]]. <br> | ||
+ | 1 % Galactose induction at the beginning. Initial O.D. 2 <br> | ||
+ | 270 rpm. <br> | ||
+ | Initial temperature 28 ºC.<br> | ||
+ | |||
+ | |||
+ | |||
+ | <html> | ||
+ | <div style=" width:55%;"><center> | ||
+ | <h2> | ||
+ | Back to<a href="https://2008.igem.org/Team:Valencia/Notebook/August"><font color="#047DB5"> August </font></a> | September | Go to <a href="https://2008.igem.org/Team:Valencia/Notebook/October"><font color="#047DB5"> October </font></a></h2> | ||
+ | </div></center> | ||
+ | </html> | ||
+ | |||
+ | |||
+ | </div> | ||
</div> | </div> |
Latest revision as of 16:56, 19 October 2008
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September 2nd
-We carried out the following experiment:
Four strains in LCCs with SP medium.
1 % Galactose induction at the beginning. Initial O.D. 0,7
275 rpm.
-Team meeting: Checking project progress.
September 3rd
-We characterized LCC temperature evolution with water.
September 5th
-We characterized LCC temperature evolution with SP medium and SP medium with bleach (to avoid contamination).
September 8th
- We observed the culture after the experiment under the microcope. There was no contamination but we did observe some elongated microorganisms, same size as S. cerevisiae.
- We discovered that our plaque with the four strains for the following experiment had suffered cross contamination.
September 9th
-We carried out the following experiment:
Four strains in LCCs with SP medium.
1 % Galactose induction at the beginning. Initial O.D. 0,6
270 rpm.
Initial temperature 31 ºC. Too high.
September 11th
-We repeated the same experiment:
Four strains in LCCs with SP medium.
1 % Galactose induction at the beginning. Initial O.D. 0,6
270 rpm.
Initial temperature 31 ºC. Too high again.
September 15th
-We repeated the same experiment:
Four strains in LCCs with SP medium.
1 % Galactose induction at the beginning. Initial O.D. 0,6
270 rpm.
Initial temperature 28 ºC. Perfect!
September 20th
-Trying to reproduce the previous experiment...
Four strains in LCCs with SP medium.
1 % Galactose induction at the beginning. Initial O.D. 0,6
270 rpm.
Initial temperature 28 ºC. But room temperature too high
September 24th
- We repeated the same experiment:
Four strains in LCCs with SP medium.
1 % Galactose induction at the beginning. Initial O.D. 0,6
270 rpm.
Initial temperature 28 ºC.
September 26th
- Team meeting: Checking project progress and inmediate future actions.
September 27th
- We tryied and increased initial O.D.
Four strains in LCCs with SP medium.
1 % Galactose induction at the beginning. Initial O.D. 2
270 rpm.
Initial temperature 28 ºC.