Brown: Team Resistance/19 October 2008

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== 19 October 2008 ==
* Miniprep of the ligation transformation of S105.  Then ran a restriction digest to verify S105 and Biobrick vector ligation.  A restriction digest was done rather than the verification PCR.  The restriction digest was done with Spe and EcoRI (Multicore).
* Miniprep of the ligation transformation of S105.  Then ran a restriction digest to verify S105 and Biobrick vector ligation.  A restriction digest was done rather than the verification PCR.  The restriction digest was done with Spe and EcoRI (Multicore).
*Restriction Digest: 5 ul Multicore, 1 ul Spe and Eco RI, 4 ul S105 Transformation Miniprep DNA (165.3 ng/ul), .5 ul BSA, 38.5 ul MilliQ water.
*Restriction Digest: 5 ul Multicore, 1 ul Spe and Eco RI, 4 ul S105 Transformation Miniprep DNA (165.3 ng/ul), .5 ul BSA, 38.5 ul MilliQ water.
*Ran a gel of the PCR products from October 18.
*Ran a gel of the PCR products from October 18.

Latest revision as of 01:24, 21 October 2008


Toxipop lab notebook.png

19 October 2008

  • Miniprep of the ligation transformation of S105. Then ran a restriction digest to verify S105 and Biobrick vector ligation. A restriction digest was done rather than the verification PCR. The restriction digest was done with Spe and EcoRI (Multicore).
  • Restriction Digest: 5 ul Multicore, 1 ul Spe and Eco RI, 4 ul S105 Transformation Miniprep DNA (165.3 ng/ul), .5 ul BSA, 38.5 ul MilliQ water.
  • Ran a gel of the PCR products from October 18.
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