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Brown: Team Resistance/19 October 2008
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| + | == 19 October 2008 == | ||
* Miniprep of the ligation transformation of S105. Then ran a restriction digest to verify S105 and Biobrick vector ligation. A restriction digest was done rather than the verification PCR. The restriction digest was done with Spe and EcoRI (Multicore). | * Miniprep of the ligation transformation of S105. Then ran a restriction digest to verify S105 and Biobrick vector ligation. A restriction digest was done rather than the verification PCR. The restriction digest was done with Spe and EcoRI (Multicore). | ||
*Restriction Digest: 5 ul Multicore, 1 ul Spe and Eco RI, 4 ul S105 Transformation Miniprep DNA (165.3 ng/ul), .5 ul BSA, 38.5 ul MilliQ water. | *Restriction Digest: 5 ul Multicore, 1 ul Spe and Eco RI, 4 ul S105 Transformation Miniprep DNA (165.3 ng/ul), .5 ul BSA, 38.5 ul MilliQ water. | ||
*Ran a gel of the PCR products from October 18. | *Ran a gel of the PCR products from October 18. | ||
Latest revision as of 01:24, 21 October 2008
19 October 2008
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