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Brown: Team Resistance/9 July 2008
From 2008.igem.org
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*OPTICAL DENSITY TEST: | *OPTICAL DENSITY TEST: | ||
*We made multiple solutions of pVJ4 and added varying amounts of Arabinose to determine how long complete lysis takes. We also added Chloramphenicol (1:1 ratio) in addition to Arabinose to see whether it would speed up lysis. | *We made multiple solutions of pVJ4 and added varying amounts of Arabinose to determine how long complete lysis takes. We also added Chloramphenicol (1:1 ratio) in addition to Arabinose to see whether it would speed up lysis. | ||
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*Initial OD 50mL culture: .038 | *Initial OD 50mL culture: .038 | ||
| - | + | .2%(1) .2%(2) .4%(1) .4%(2) .2% + Chloramphenicol(1) .2% + Chloramphenicol(2) Control | |
| - | + | 1 .084 .085 .079 .079 .062 .053 .073 | |
| - | + | 2 .065 .069 .040 .048 .044 .047 .076 | |
| - | + | 3 .018 .018 .023 .021 .034 .050 .075 | |
| - | + | 4 .014 .015 .013 .013 .050 .044 .071 | |
*The first few readings indicated an increase in the optical density. This is to be expected because the bacteria are in the mid-log phase and still growing. Growth must occur in order for the S, R, and Rz genes to be expressed and translated. Cell lysis is complete in four hours. | *The first few readings indicated an increase in the optical density. This is to be expected because the bacteria are in the mid-log phase and still growing. Growth must occur in order for the S, R, and Rz genes to be expressed and translated. Cell lysis is complete in four hours. | ||
*The addition of Chloramphenicol did not alter the rate of lysis. | *The addition of Chloramphenicol did not alter the rate of lysis. | ||
Latest revision as of 01:27, 21 October 2008
9 July 2008
.2%(1) .2%(2) .4%(1) .4%(2) .2% + Chloramphenicol(1) .2% + Chloramphenicol(2) Control 1 .084 .085 .079 .079 .062 .053 .073 2 .065 .069 .040 .048 .044 .047 .076 3 .018 .018 .023 .021 .034 .050 .075 4 .014 .015 .013 .013 .050 .044 .071
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