Team:Chiba/Calendar-Home/20 August 2008

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(Difference between revisions)
(→Team:Communication)
(→Team:Input)
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*[http://partsregistry.org/Part:BBa_J06650 BBa_J06650](2006)
*[http://partsregistry.org/Part:BBa_J06650 BBa_J06650](2006)
*[http://partsregistry.org/Part:BBa_J22136 BBa_J22136](2007)
*[http://partsregistry.org/Part:BBa_J22136 BBa_J22136](2007)
-
*[http://partsregistry.org/Part:BBa_J22141 BBa_J22141](2007)
+
*[http://partsregistry.org/Part:BBa_J22141 BBa_J22141](2007)-->only one colony
-->BBa_J22141のみコロニーがほとんど生えていなかった。(１個のみ)
-->BBa_J22141のみコロニーがほとんど生えていなかった。(１個のみ)
-
 
-
 
===Team:Communication===
===Team:Communication===

Revision as of 08:53, 24 October 2008

>Home | Notebook

19 August 2008 <|> 21 August 2008

Contents

Laboratory work

Team:Input

PCR

  • [http://partsregistry.org/Part:BBa_J22136 BBa_J22136](2007)①
  • [http://partsregistry.org/Part:BBa_J22136 BBa_J22136](2008)②
  • [http://partsregistry.org/Part:BBa_J22141 BBa_J22141](2007)③
  • [http://partsregistry.org/Part:BBa_J22141 BBa_J22141](2008)④
  • [http://partsregistry.org/Part:BBa_E0612 BBa_E0612](2008)⑤
>
Sample No. ①②③④⑤
DNA tamplate 11111
FW primer 55555
VR primer 55555
dNTPmix 1010101010
thermo pol buffer 1010101010
DNA pol(VENT) 11111
dH2O 6868686868
TOTAL 100100100100100

-->Gel Check


>
Sample No. ①②③④⑤
Sample DNA 11111
loading dye 11111
dH2O 44444
Total 66666

-->どれもバンド出ず。

Transformation

  • [http://partsregistry.org/Part:BBa_R0051 BBa_R0051](2007)
  • [http://partsregistry.org/Part:BBa_R0051 BBa_R0051](2006)
  • [http://partsregistry.org/Part:BBa_J06650 BBa_J06650](2007)
  • [http://partsregistry.org/Part:BBa_J06650 BBa_J06650](2006)
  • [http://partsregistry.org/Part:BBa_J22136 BBa_J22136](2007)
  • [http://partsregistry.org/Part:BBa_J22141 BBa_J22141](2007)-->only one colony

-->BBa_J22141のみコロニーがほとんど生えていなかった。(１個のみ)

Team:Communication

(18/8)--->Mini prep
  • [http://partsregistry.org/Part:BBa_C0070 BBa_C0070](2007)
  • [http://partsregistry.org/Part:BBa_C0070 BBa_C0070](2006)
  • [http://partsregistry.org/Part:BBa_C0076 BBa_C0076](2007)
  • [http://partsregistry.org/Part:BBa_C0078 BBa_C0078](2007)
  • [http://partsregistry.org/Part:BBa_C0078 BBa_C0078](2006)
  • [http://partsregistry.org/Part:BBa_C0170 BBa_C0170](2007)
  • [http://partsregistry.org/Part:BBa_C0170 BBa_C0170](2006)
  • [http://partsregistry.org/Part:BBa_C0178 BBa_C0178](2007)
  • [http://partsregistry.org/Part:BBa_C0178 BBa_C0178](2006)


--->Gel Check
Chiba-0820-3.JPG
Sample DNA 3
Loading Dye 2
dH2O 7
TOTAL 12μL
From left;
  • [http://partsregistry.org/Part:BBa_C0076 BBa_C0076](2007) -> None
  • [http://partsregistry.org/Part:BBa_C0078 BBa_C0078](2007) -> None
  • [http://partsregistry.org/Part:BBa_C0078 BBa_C0078](2006) -> None


Chiba-0820-4.JPG
Sample DNA 10
Loading Dye 4
dH2O 10
TOTAL 24μL
From left;
  • [http://partsregistry.org/Part:BBa_C0078 BBa_C0078](2007) -> None
  • [http://partsregistry.org/Part:BBa_C0078 BBa_C0078](2006) -> OK
  • [http://partsregistry.org/Part:BBa_C0076 BBa_C0076](2007) -> None
  • [http://partsregistry.org/Part:BBa_C0070 BBa_C0070](2007) -> OK
  • [http://partsregistry.org/Part:BBa_C0070 BBa_C0070](2006) -> OK



Chiba-0820.JPG
Sample DNA 3
Loading Dye 2
dH2O 7
TOTAL 12μL
From left;
  • [http://partsregistry.org/Part:BBa_C0170 BBa_C0170](2007) -> OK
  • [http://partsregistry.org/Part:BBa_C0170 BBa_C0170](2006) -> OK
  • [http://partsregistry.org/Part:BBa_C0178 BBa_C0178](2007) -> OK
  • [http://partsregistry.org/Part:BBa_C0178 BBa_C0178](2006) -> OK


--->Digestion test

  • [http://partsregistry.org/Part:BBa_C0170 BBa_C0170](2007)
  • [http://partsregistry.org/Part:BBa_C0170 BBa_C0170](2006)
  • [http://partsregistry.org/Part:BBa_C0178 BBa_C0178](2007)
  • [http://partsregistry.org/Part:BBa_C0178 BBa_C0178](2006)
Sample No 12345678
[http://partsregistry.org/Part:BBa_C0170 BBa_C0170](2006) 3---3---
[http://partsregistry.org/Part:BBa_C0170 BBa_C0170](2007) -3---3--
[http://partsregistry.org/Part:BBa_C0178 BBa_C0178](2006) --3---3-
[http://partsregistry.org/Part:BBa_C0178 BBa_C0178](2007) ---3---3
EcoRⅠ 0.10.10.10.10.10.10.10.1
PstⅠ ----0.10.10.10.1
Buffer EcoRⅠ 11111111
BSA 11111111
dH2O 4.94.94.94.94.84.84.84.8
TOTAL 1010101010101010


--->Gel Check
Chiba-0820-2.JPG
Sample DNA 3
Loading Dye 2
dH2O 7
TOTAL 12μL
From left;
(Single Digestion 1~4)
  • [http://partsregistry.org/Part:BBa_C0170 BBa_C0170](2006) -> OK
  • [http://partsregistry.org/Part:BBa_C0170 BBa_C0170](2007) -> OK
  • [http://partsregistry.org/Part:BBa_C0178 BBa_C0178](2006) -> OK
  • [http://partsregistry.org/Part:BBa_C0178 BBa_C0178](2007) -> OK
(Double Digestion 1~4)
  • [http://partsregistry.org/Part:BBa_C0170 BBa_C0170](2006) -> OK
  • [http://partsregistry.org/Part:BBa_C0170 BBa_C0170](2007) -> OK
  • [http://partsregistry.org/Part:BBa_C0178 BBa_C0178](2006) -> OK
  • [http://partsregistry.org/Part:BBa_C0178 BBa_C0178](2007) -> OK

Team:Output

Transformation

  • [http://partsregistry.org/Part:BBa_J52008 BBa_J52008](Luciferase Renilla)-->colony
  • [http://partsregistry.org/Part:BBa_I712019 BBa_I712019](Luciferase Fire fly)-->no colony
  • [http://partsregistry.org/Part:BBa_J33202 BBa_J33202](LacZ)-->colony
  • [http://partsregistry.org/Part:BBa_E2030 BBa_E2030](Venus YFP)-->not so good
  • YFP(P-GEX)-->colony
  • [http://partsregistry.org/Part:BBa_F2620 BBa_F2620]-->colony
  • [http://partsregistry.org/Part:BBa_T9002 BBa_T9002]-->colony


Luciferase(J52008:2007,2008 I712019:2008)(Amp)
Lac Z(J33202:2007,2008)(Amp)
Venus(E2030:2007,2008)(Kam)
Craig Venus(Amp?Kan?)
T9002(Amp)
F2620(Amp)



luciferase,LacZ,Venus
competent cell 50μl
DNA 1μl
soc 200μl
plating 250μl


Craig Venus,T9002,F2620
competent cell 30μl
DNA 1μl
soc 120μl
plating 20μl


result

成功→Luciferase(J52008:2007),Lac Z(J33202:2007),Craig Venus,T9002,F2620
失敗→Luciferase(J52008:2008 J52008:2008),Lac Z(J33202:2008),Venus(J32007:2007,2008)
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