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Team:Chiba/Calendar-Home/10 September 2008
From 2008.igem.org
(Difference between revisions)
(New page: >Home | Notebook 9 September 2008 <|> 11 September 2008...) |
(→Team:Communication) |
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:<table width="315" border="2" cellpadding="0" cellspacing="0" bordercolor="#000000"> | :<table width="315" border="2" cellpadding="0" cellspacing="0" bordercolor="#000000"> | ||
<tr> | <tr> | ||
| - | <td width="257">DNA Template</td> | + | <td width="257">DNA Template(μL)</td> |
<td>1</td> | <td>1</td> | ||
</tr> | </tr> | ||
<tr> | <tr> | ||
| - | <td>dNTP mix</td> | + | <td>dNTP mix(μL)</td> |
<td>5</td> | <td>5</td> | ||
</tr> | </tr> | ||
<tr> | <tr> | ||
| - | <td>Foward Primer</td> | + | <td>Foward Primer(μL)</td> |
<td>0.3</td> | <td>0.3</td> | ||
</tr> | </tr> | ||
<tr> | <tr> | ||
| - | <td>Reverse Primer</td> | + | <td>Reverse Primer(μL)</td> |
<td>0.3</td> | <td>0.3</td> | ||
</tr> | </tr> | ||
<tr> | <tr> | ||
| - | <td>taq DNA polymerase</td> | + | <td>taq DNA polymerase(μL)</td> |
<td>0.5</td> | <td>0.5</td> | ||
</tr> | </tr> | ||
<tr> | <tr> | ||
| - | <td>Thermopol Buffer</td> | + | <td>Thermopol Buffer(μL)</td> |
<td>3</td> | <td>3</td> | ||
</tr> | </tr> | ||
<tr> | <tr> | ||
| - | <td>Nuclease free water</td> | + | <td>Nuclease free water(μL)</td> |
<td>20</td> | <td>20</td> | ||
</tr> | </tr> | ||
<tr> | <tr> | ||
| - | <td>TOTAL</td> | + | <td>TOTAL(μL)</td> |
| - | <td> | + | <td>30</td> |
</tr> | </tr> | ||
</table> | </table> | ||
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--->(28/8)'''[[Team:Chiba/protocol/DNA Purification/sigma|Miniprep]]''' | --->(28/8)'''[[Team:Chiba/protocol/DNA Purification/sigma|Miniprep]]''' | ||
| - | + | eluted with 50μL of TE buffer. | |
| - | + | ||
===Team:Output=== | ===Team:Output=== | ||
Revision as of 02:13, 25 October 2008
9 September 2008 <|> 11 September 2008
Contents |
Laboratory work
Team:Input
no work
Team:Communication
(9/9)-->
- Colony Count
- LuxI
- LuxI with LVA
- Las
- Background(R0010)
- Background(C0261)
Pick colony
- Colony PCR of 10 colonies from ligation plate(9/9-(2)) and 3 colonies from ligation plate(9/9-(3)) one from control plate(BBa_F2620[http://partsregistry.org/Part:BBa_F2620](2007)).
DNA Template(μL) 1 dNTP mix(μL) 5 Foward Primer(μL) 0.3 Reverse Primer(μL) 0.3 taq DNA polymerase(μL) 0.5 Thermopol Buffer(μL) 3 Nuclease free water(μL) 20 TOTAL(μL) 30 ??
- 95℃,5min -> ( 95℃,1min -> 52℃,1min -> 72℃,1min )･･･25cycles -> 72℃,10min -> 6℃
??
-->Gel Check
--->(28/8)Miniprep
eluted with 50μL of TE buffer.
Team:Output
-->Gel check
-->Mini prep
