Team:Chiba/Calendar-Home/23 September 2008

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*[[Team:Chiba/protocol/phenotype/timedelay/j|time-delay test]]
*[[Team:Chiba/protocol/phenotype/timedelay/j|time-delay test]]
**conditions
**conditions
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#strain
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#strain<br>Senders:XL10GOLD
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<br>Receiver:JW1908<br>
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Senders:XL10GOLD
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Receiver:JW1908
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#temparature<br>37°C and 30°C
#temparature<br>37°C and 30°C

Revision as of 04:54, 25 October 2008

>Home | Notebook

22 September 2008 <|> 24 September 2008

Laboratory work

Team:Communication

  1. strain
    Senders:XL10GOLD


Receiver:JW1908

  1. temparature
    37°C and 30°C

Team:Output


Digestion test
BBa_E0240

DNA 2
EcoRⅠ 0.5
PstⅠ 0.5
BSA(x10) 1
EcoRⅠ Buffer(x10) 1
dH2O 5
TOTAL 10


TRANSFORMATION
BBa_I6107
BBa_I6206
BBa_I6101
BBa_I6042
BBa_I0462



Digestion
Vector:BBa_R0062①
Insert:BBa_E0240②
Vector:BBa_I7100③
Insert:BBa_R0062④
Insert:BBa_J37033⑤

</tr>

Sample No ①②③④⑤
DNA 2525252525
EcoRⅠ --11-
SpeⅠ 1-11-
PstⅠ 111-1
XbaⅠ -1--1
BSA(x10) 444-4
EcoRⅠ Buffer(x10) --44-
Buffer 2 4----
Buffer 3 -4--4
dH2O 55595
TOTAL 4040404040


->1hour 37℃
->SAP 1μl(Vector only) ->1hour 37℃ ->30min 65℃
->Zymo ->10μl
Gel cheack

DNA 1
Loading Dye 1
dH2O 4
TOTAL 6


->③&④&⑤のLigationはしない

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