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Team:Chiba/protocol/Time Delay Test
From 2008.igem.org
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#Inoculated them independently in liquid media. Incubated at 37℃ 12h. | #Inoculated them independently in liquid media. Incubated at 37℃ 12h. | ||
Revision as of 05:57, 26 October 2008
- Transformed sender and receiver into E coli strains.
- Inoculated them independently in liquid media. Incubated at 37℃ 12h.
- Inoculated again in Fresh liquid media upto about OD600=2 at 37℃
- Washed sender and receiver.
- Mixed them. (Sender:Receiver=1000μL:1000μL)
- Incubated at 30°C.
- Measured intensity of green fluorescence at regular time intervals.(Fluoroskan AscentR FL＆Fluoroskan AscentR Thermo ELECTRON CORPORATION)
