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From 2008.igem.org

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Contents 1 Transformation 1.1 Zymo comp. 1.2 Electro poration 2 DNA Purification 2.1 Sigma prep 2.2 Zymo DNA Cleam&Concentrator Kit 3 Agarose gel electrophoresis 4 Digestion 5 PCR 6 Ligation 6.1 Gel extract 6.2 Dephosphorylation of DNA 6.3 Phosphorylation of DNA 6.4 Ligation 7 Time Delay Test

Transformation

Zymo comp.

Electro poration

DNA Purification

Sigma prep

Zymo DNA Cleam&Concentrator Kit

Agarose gel electrophoresis

Digestion

PCR

Ligation

Gel extract

Dephosphorylation of DNA

Phosphorylation of DNA

Ligation

Time Delay Test

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