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Team:Chiba/protocol/Time Delay Test
From 2008.igem.org
(Difference between revisions)
| Line 8: | Line 8: | ||
#Washed sender and receiver. | #Washed sender and receiver. | ||
#Mixed them. (Sender:Receiver=1000μL:1000μL) | #Mixed them. (Sender:Receiver=1000μL:1000μL) | ||
| - | #Incubated at | + | #Incubated at 25°C, 30°C or 37°C. |
#Measured intensity of green fluorescence at regular time intervals.(Fluoroskan Ascent<sup>R</sup> FL＆Fluoroskan Ascent<sup>R</sup> Thermo ELECTRON CORPORATION) | #Measured intensity of green fluorescence at regular time intervals.(Fluoroskan Ascent<sup>R</sup> FL＆Fluoroskan Ascent<sup>R</sup> Thermo ELECTRON CORPORATION) | ||
Latest revision as of 06:38, 26 October 2008
Time Delay Test
- Transformed sender and receiver into E coli strains.
- Inoculated them independently in liquid media. Incubated at 37℃ 12h.
- Inoculated again in Fresh liquid media upto about OD600=2 at 37℃
- Washed sender and receiver.
- Mixed them. (Sender:Receiver=1000μL:1000μL)
- Incubated at 25°C, 30°C or 37°C.
- Measured intensity of green fluorescence at regular time intervals.(Fluoroskan AscentR FL＆Fluoroskan AscentR Thermo ELECTRON CORPORATION)
