Team:Heidelberg/Notebook/Sensing Group/Notebook/3rdweek

From 2008.igem.org

(Difference between revisions)
(Monday, 08/18/2008)
(Tuesday, 08/19/2008)
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== Tuesday, 08/19/2008 ==
== Tuesday, 08/19/2008 ==
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* Miniprep of pDK48 and LuxS
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* gel of Fusion constructs and gelextraction of pDK48 from yesterday
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[[Image:HD 080819-Fusion PCR pDK48-Digestion.png|center|thumbs|500px|Fusion PCR and pDK48 gel extraction]]
== Wednesday, 08/20/2008 ==
== Wednesday, 08/20/2008 ==

Revision as of 14:25, 26 October 2008

Back to the overview

Contents

Monday, 08/18/2008

  • PCR for LuxQ-1 and LuxQ-2 for Fusion constructs. Different Mg concentrations between 1-4 % were used. For LuxQ-2 another PCR was run using as template V. harveyi colonies
PCR of LuxQ-1 and LuxQ-2 for subsequent fusion PCR
  • Fusion-PCR for Fusion-1 and Fusion-2, each 4 tubes (2 µl Tar, 1 µl LuxQ)
  • preparation of O/N culture of LuxS and pDK48 transformed cells for subsequent miniprep
  • digestion of pDK48 with NcoI/NdeI
  • digestion of pTrc99alpha and LuxQ (with BamHI and NcoI) and gelextraction

Tuesday, 08/19/2008

  • Miniprep of pDK48 and LuxS
  • gel of Fusion constructs and gelextraction of pDK48 from yesterday
Fusion PCR and pDK48 gel extraction

Wednesday, 08/20/2008

Thursday, 08/21/2008

Friday, 08/22/2008

Go to 4th week