Team:Warsaw/Calendar-Main/11 June 2008

(Difference between revisions)

Latest revision as of 14:49, 26 October 2008


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Transformation of E. coli TOP10 carrying pZC320 with:
- pMPMT5,
- pMPMT5-AID,
- pMPMT5-AIDT7,
- pMPMT5-AID+T7.
Plating transformants on 'TAXI' (LB + Tetracycline + IPTG + Ampicillin 30 μg/mL + X-Gal).
Inoculation of transformants to liquid LB + Tetracycline + Ampicillin 30 μg/mL +/- 0.1% Ara.

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-<h3>Michał L., Ewa, Marcin</h3>
+<html>
+<h3> Blue/white and rifampicin test #1</h3><h4>Michał L., Ewa, Piotr</h4>
+<ol>
+<li><a href="https://2008.igem.org/wiki/index.php?title=Wiki/Team:Warsaw/protocols#chemotransform">Transformation</a> of <i>E. coli</i> <a href="https://2008.igem.org/Wiki/Team:Warsaw/igem_notebook.htm#top10">TOP10</a> carrying <a href="https://2008.igem.org/Wiki/Team:Warsaw/vectors/pZC320">pZC320</a> with:
+<ul><li><a href=https://2008.igem.org/Wiki/Team:Warsaw/vectors/pMPM-T5-omega>pMPMT5</a>,</li>
+<li><a href=https://2008.igem.org/Wiki/Team:Warsaw/vectors/pMPM-T5-AID>pMPMT5-AID</a>,</li>
+<li><a href=https://2008.igem.org/Wiki/Team:Warsaw/vectors/pMPM-T5%2BAIDT7>pMPMT5-AIDT7</a>, </li>
+<li><a href=https://2008.igem.org/Wiki/Team:Warsaw/vectors/pMPM-T5%2BAID%2BT7>pMPMT5-AID+T7</a>.</li></ul>
+<li>Plating transformants on 'TAXI' (LB + Tetracycline + IPTG + Ampicillin 30 μg/mL + X-Gal).</li>
-<p>Transformation of E.coli Top10 carrying pZC320 with pMPMT5, pMPMT5-AID, pMPMT5-AIDT7 or pMPMT5-AiD+T7.
-Plating transformants on 'TAXI' (LB + Tetracycline + IPTG + Ampicillin 30 μg/mL + X-Gal).</p>
-Rifampicin test of transformants
+<li>Inoculation of transformants to liquid LB + Tetracycline + Ampicillin 30 μg/mL +/- 0.1% Ara. </li>
+</ol>
+</html>