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Team:Heidelberg/Notebook/Sensing Group/Notebook/4thweek
From 2008.igem.org
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== Monday, 08/25/2008 == | == Monday, 08/25/2008 == | ||
| - | [[Image:HD 080826-pDK48 digestions.png|right|thumb| | + | [[Image:HD 080826-pDK48 digestions.png|right|thumb|200px|Digestion of pDK48 with xbaI and PstI]] |
* sequentiell and simultaneous digestion of pDk48 NcoI/NdeI and NcoI/KpnI | * sequentiell and simultaneous digestion of pDk48 NcoI/NdeI and NcoI/KpnI | ||
--> not successful | --> not successful | ||
* preparation of O/N culture of pDK48 transformed cells | * preparation of O/N culture of pDK48 transformed cells | ||
* digestion of pDK48 with XbaI and PstI to check wether the vector is the right one | * digestion of pDK48 with XbaI and PstI to check wether the vector is the right one | ||
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| + | <br style="clear: both;"/> | ||
== Tuesday, 08/26/2008 == | == Tuesday, 08/26/2008 == | ||
Revision as of 14:52, 26 October 2008
Contents |
Monday, 08/25/2008
- sequentiell and simultaneous digestion of pDk48 NcoI/NdeI and NcoI/KpnI
--> not successful
- preparation of O/N culture of pDK48 transformed cells
- digestion of pDK48 with XbaI and PstI to check wether the vector is the right one
