Team:Heidelberg/Notebook/Sensing Group/Notebook/4thweek

From 2008.igem.org

(Difference between revisions)
(Monday, 08/25/2008)
(Tuesday, 08/26/2008)
Line 129: Line 129:
== Tuesday, 08/26/2008 ==
== Tuesday, 08/26/2008 ==
 +
[[Image:HD 080826-pDK48 digestions.png|right|thumb|200px|Digestion of pDK48 with xbaI and PstI yield expected results]]
* digestion of pDK48 with XbaI and PstI to check wether the vector is the right one
* digestion of pDK48 with XbaI and PstI to check wether the vector is the right one

Revision as of 14:56, 26 October 2008

Back to the overview

Contents

Monday, 08/25/2008

  • sequentiell and simultaneous digestion of pDk48 NcoI/NdeI and NcoI/KpnI

--> not successful

  • preparation of O/N culture of pDK48 transformed cells

Tuesday, 08/26/2008

Digestion of pDK48 with xbaI and PstI yield expected results
  • digestion of pDK48 with XbaI and PstI to check wether the vector is the right one


Wednesday, 08/27/2008

Thursday, 08/28/2008

Friday, 08/29/2008

Go to 5th week