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Team:Heidelberg/Notebook/Sensing Group/Notebook/4thweek
From 2008.igem.org
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[[Image:HD 080826-pDK48 digestions.png|right|thumb|200px|Digestion of pDK48 with xbaI and PstI yield expected results]] | [[Image:HD 080826-pDK48 digestions.png|right|thumb|200px|Digestion of pDK48 with xbaI and PstI yield expected results]] | ||
* digestion of pDK48 with XbaI and PstI to check wether the vector is the right one | * digestion of pDK48 with XbaI and PstI to check wether the vector is the right one | ||
| + | * digestion of Fusion-1 (from 08/21/2008) with NcoI/NdeI, with subsequent gelextraction | ||
| + | * Ligation of Fusion-1 and pDk48 and transformation into DH5a competent cells | ||
=== LuxS Test === | === LuxS Test === | ||
Revision as of 15:02, 26 October 2008
Contents |
Monday, 08/25/2008
- sequentiell and simultaneous digestion of pDk48 NcoI/NdeI and NcoI/KpnI
--> not successful
- preparation of O/N culture of pDK48 transformed cells
Tuesday, 08/26/2008
Cloning
- digestion of pDK48 with XbaI and PstI to check wether the vector is the right one
- digestion of Fusion-1 (from 08/21/2008) with NcoI/NdeI, with subsequent gelextraction
- Ligation of Fusion-1 and pDk48 and transformation into DH5a competent cells
LuxS Test
