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Team:Heidelberg/Notebook/Visualization/Notebook/1stweek
From 2008.igem.org
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==Monday 01/09/2008== | ==Monday 01/09/2008== | ||
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| + | * Preparation of TB media with an Agar concentration of 0.4 %, 0.2 % and 0.1 %. | ||
| + | |||
| + | First microscope test using MG1655 in 6 small chambers from Labtek: | ||
| + | |||
| + | 3 types: | ||
| + | I - 0.1% agar in LB | ||
| + | II - 0.25% agar in LB | ||
| + | III - 0.5% agar in LB | ||
| + | |||
| + | View: | ||
| + | I II III | ||
| + | I II III | ||
| + | |||
| + | * In I and II each chamber was filled with just 100 µl LB agar to cover the whole ground. | ||
| + | |||
| + | * Because the III. solution was more viscous 150 µl were necessary to cover the ground. | ||
| + | |||
| + | * The bacteria were put into one corner of a chamber. (2 µl of a MG1655 overnight culture) | ||
| + | They have been set under the media or in between. | ||
| + | |||
| + | * About that another 100 µl of a LB solution with 2 % agar were poured. After the second layer has solidified further 250 µl LB-media were added. | ||
| + | |||
| + | * The chambers were incubated overnight at 37 °C. For microscope on the next day. | ||
| + | |||
| + | Notes: Because of surface tensions the solutions tend to be thicker at the borders of the chamber than in the center. | ||
| + | -> In the further future 150 - 200 µl will be used. | ||
| + | |||
==Tuesday 02/09/2008== | ==Tuesday 02/09/2008== | ||
==Wednesday 03/09/2008== | ==Wednesday 03/09/2008== | ||
Revision as of 17:00, 26 October 2008
Contents |
First week
Monday 01/09/2008
- Preparation of TB media with an Agar concentration of 0.4 %, 0.2 % and 0.1 %.
First microscope test using MG1655 in 6 small chambers from Labtek:
3 types: I - 0.1% agar in LB II - 0.25% agar in LB III - 0.5% agar in LB
View: I II III I II III
- In I and II each chamber was filled with just 100 µl LB agar to cover the whole ground.
- Because the III. solution was more viscous 150 µl were necessary to cover the ground.
- The bacteria were put into one corner of a chamber. (2 µl of a MG1655 overnight culture)
They have been set under the media or in between.
- About that another 100 µl of a LB solution with 2 % agar were poured. After the second layer has solidified further 250 µl LB-media were added.
- The chambers were incubated overnight at 37 °C. For microscope on the next day.
Notes: Because of surface tensions the solutions tend to be thicker at the borders of the chamber than in the center. -> In the further future 150 - 200 µl will be used.
