Team:Paris/Modeling/Workflow Example
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{{Paris/Header|Generic Workflow on an Example}} | {{Paris/Header|Generic Workflow on an Example}} | ||
- | Here, we will explain the generic protocol that leads to the characterisation of one of the steps of our system. The choosen example is '''the | + | Here, we will explain the generic protocol that leads to the characterisation of one of the steps of our system. The choosen example is '''the ACTIVITY of the PROMOTER pFliL''' (that leads to the production of the '''Fluorescent Protein FP1''') '''in function of the TRANSCRIPTION FACTORS FlhDC and FliA'''. |
We know that ''the hexamere FlhD<sub>4</sub>C<sub>2</sub>'' and the protein ''FliA'' act both on the promoter ''pFliL'' as inducers... how do we evaluate this phenomenon? <br> | We know that ''the hexamere FlhD<sub>4</sub>C<sub>2</sub>'' and the protein ''FliA'' act both on the promoter ''pFliL'' as inducers... how do we evaluate this phenomenon? <br> | ||
Starting from [[Team:Paris/Modeling/Molecular_Reactions|molecular reactions]] '''(1)''', we develop a [[Team:Paris/Modeling/FromMolReactToNLOde|model of our system]] '''(2)''', and then we identify the parameters '''(3)''' that needs to be estimated. Essentially, it amounts to estimate [[Team:Paris/Modeling/Protocol_Of_Characterisation|Promoters Activities as functions of their Transcription Factors]]. To do so, we propose [[Team:Paris/Modeling/Protocol_Of_Characterisation|constructs]] '''(4)''', [[Team:Paris/Modeling/Protocol_Of_Characterisation|protocols]] '''(5)''' and [[Team:Paris/Modeling/Implementation|programs]] '''(6)'''. | Starting from [[Team:Paris/Modeling/Molecular_Reactions|molecular reactions]] '''(1)''', we develop a [[Team:Paris/Modeling/FromMolReactToNLOde|model of our system]] '''(2)''', and then we identify the parameters '''(3)''' that needs to be estimated. Essentially, it amounts to estimate [[Team:Paris/Modeling/Protocol_Of_Characterisation|Promoters Activities as functions of their Transcription Factors]]. To do so, we propose [[Team:Paris/Modeling/Protocol_Of_Characterisation|constructs]] '''(4)''', [[Team:Paris/Modeling/Protocol_Of_Characterisation|protocols]] '''(5)''' and [[Team:Paris/Modeling/Implementation|programs]] '''(6)'''. |
Revision as of 19:55, 26 October 2008
Generic Workflow on an Example
Here, we will explain the generic protocol that leads to the characterisation of one of the steps of our system. The choosen example is the ACTIVITY of the PROMOTER pFliL (that leads to the production of the Fluorescent Protein FP1) in function of the TRANSCRIPTION FACTORS FlhDC and FliA.
We know that the hexamere FlhD4C2 and the protein FliA act both on the promoter pFliL as inducers... how do we evaluate this phenomenon?
Once we get every parameters for every functions involved in our Full System, we have got a "Modular Virtual Lab" of our System that aims to be predictive . |