Team:Warsaw/Calendar-Main/1 September 2008

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Purification of proteins: A-alpha

Piotr

Liquid LB with chloramphenicol and ampicillin was inoculated with Rosetta strain carrying pET15b+A_alpha.

Blue-white screening and rifampicin test in GM2163

Michał L., Ewa

Blue colonies of Top10 strains transformed with pZC320 from various GM2163 strains were counted.
Results (% of blue colonies):
pMPMT5-omega 100%
pMPMT5-AID 100%
pMPMT5-AIDT7 50%
pMPMT5-AID+T7 0% (weird)

Results of rifampicin test

Strain	Inducer / repressor	Number of colonies on rifampicin
GM2163	none	1
GM2163 pMPM-AID	0.1% Arabinose	47
GM2163 pMPM-AID	none	4
GM2163 pMPM-AID+T7	0.1% Arabinose	99
GM2163 pMPM-AID+T7	none	2
GM2163 pMPM-AIDT7	0.1% Arabinose	12
GM2163 pMPM-AIDT7	none	9

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+<html>
+<h3>Purification of proteins: A-alpha</h3><h4>Piotr</h4>
-<p><ol>
+<p>Liquid LB with chloramphenicol and ampicillin was inoculated with <a href="https://2008.igem.org/Wiki/Team:Warsaw/igem_notebook.htm#rosetta">Rosetta</a> strain carrying <a href=https://2008.igem.org/Wiki/Team:Warsaw/vectors/pET15b%2BA-alpha>pET15b+A_alpha</a>.
-<li>Digestion of A_geneart and pET15b+Z_alpha with NdeI and NotI, dephosphorylation of pET15b+Z_alpha  with CIAP. </li>
+</p>
-<li> Gel electrophoresis and isolation of proper bands< (450 bp - A_geneart lane and.. )</li></ol></p>
+<h3>Blue-white screening and rifampicin test in <a href="https://2008.igem.org/Wiki/Team:Warsaw/igem_notebook.htm#gm">GM2163</a></h3>
+<h4>Michał L., Ewa</h4>
+<p>
+<p>Blue colonies of Top10 strains transformed with pZC320 from various GM2163 strains were counted.<br>
+Results (% of blue colonies):<br>
+<a href=https://2008.igem.org/Wiki/Team:Warsaw/vectors/pMPM-T5-omega>pMPMT5-omega</a> 100%<br>
+<a href=https://2008.igem.org/Wiki/Team:Warsaw/vectors/pMPM-T5-AID>pMPMT5-AID</a> 100%<br>
+<a href=https://2008.igem.org/Wiki/Team:Warsaw/vectors/pMPM-T5%2BAIDT7>pMPMT5-AIDT7</a> 50%<br>
+<a href=https://2008.igem.org/Wiki/Team:Warsaw/vectors/pMPM-T5%2BAID%2BT7>pMPMT5-AID+T7</a> 0% (weird)<br>
+</p>
+<p>
+<table id="result">
+<thead><b> Results of rifampicin test</b><br></thead>
+<tr><th>Strain</th><th>Inducer / repressor</th><th>Number of colonies on rifampicin</th></tr>
+<tr><th>GM2163</th><td>none</td><td>1</td></tr>
+<tr><th>GM2163 pMPM-AID</th><td>0.1% Arabinose</td><td>47</td></tr>
+<tr><th>GM2163 pMPM-AID</th><td>none</td><td>4</td></tr>
+<tr><th>GM2163 pMPM-AID+T7</th><td>0.1% Arabinose</td><td>99</td></tr>
+<tr><th>GM2163 pMPM-AID+T7</th><td>none</td><td>2</td></tr>
+<tr><th>GM2163 pMPM-AIDT7</th><td>0.1% Arabinose</td><td>12</td></tr>
+<tr><th>GM2163 pMPM-AIDT7</th><td>none</td><td>9</td></tr>
+</table>
+</p>
+</html>
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