Team:Warsaw/Calendar-Main/1 September 2008

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<h3>Purification of proteins: A-alpha</h3><h4>Piotr</h4>
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<h3>Purification of proteins: A-alpha</h3><h4>Piotr</h4>
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<p><a href=https://2008.igem.org/Wiki/Team:Warsaw/vectors/pET15b%2BA-alpha>pET15b+A_alpha</a> in <a href="https://2008.igem.org/Wiki/Team:Warsaw/igem_notebook.htm#rosetta">Rosetta</a> strain inoculated in liquid LB with chloramphenicol and ampicillin.  
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<p>Liquid LB with chloramphenicol and ampicillin was inoculated with <a href="https://2008.igem.org/Wiki/Team:Warsaw/igem_notebook.htm#rosetta">Rosetta</a> strain carrying <a href=https://2008.igem.org/Wiki/Team:Warsaw/vectors/pET15b%2BA-alpha>pET15b+A_alpha</a>.  
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<h3>Blue-white screening and rifampicin test in <a href="https://2008.igem.org/Wiki/Team:Warsaw/igem_notebook.htm#gm">GM2163</a> - results</h3>
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<h3>Blue-white screening and rifampicin test in <a href="https://2008.igem.org/Wiki/Team:Warsaw/igem_notebook.htm#gm">GM2163</a></h3>
<h4>Michał L., Ewa</h4>
<h4>Michał L., Ewa</h4>
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Latest revision as of 21:01, 26 October 2008

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Purification of proteins: A-alpha

Piotr

Liquid LB with chloramphenicol and ampicillin was inoculated with Rosetta strain carrying pET15b+A_alpha.

Blue-white screening and rifampicin test in GM2163

Michał L., Ewa

Blue colonies of Top10 strains transformed with pZC320 from various GM2163 strains were counted.
Results (% of blue colonies):
pMPMT5-omega 100%
pMPMT5-AID 100%
pMPMT5-AIDT7 50%
pMPMT5-AID+T7 0% (weird)

Results of rifampicin test
StrainInducer / repressorNumber of colonies on rifampicin
GM2163none1
GM2163 pMPM-AID0.1% Arabinose47
GM2163 pMPM-AIDnone4
GM2163 pMPM-AID+T70.1% Arabinose99
GM2163 pMPM-AID+T7none2
GM2163 pMPM-AIDT70.1% Arabinose12
GM2163 pMPM-AIDT7none9