Team:Warsaw/Calendar-Main/17 October 2008

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<h3>Preparation of pACYC177 + OmpA_omega_</h3>
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<h4>Michał K.</h4>
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<ol><li><a href=https://2008.igem.org/wiki/index.php?title=Wiki/Team:Warsaw/protocols#plasmid_DNA_isolation>Isolation</a> of plasmids from cultures inoculated on previous day pACYC177 + OmpA_omega_.</li>
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<li>Control <a href=https://2008.igem.org/wiki/index.php?title=Wiki/Team:Warsaw/protocols#digest>digest</a> of isolated plasmids with EcoRI and PstI (Orange buffer). Gel electrophoresis - no proper clones found.</li></ol>
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<h3>Preparation of BioBricks</h3>
<h3>Preparation of BioBricks</h3>
<h4>Michał K.</h4>
<h4>Michał K.</h4>
<ol>
<ol>
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<li><a href=https://2008.igem.org/wiki/index.php?title=Wiki/Team:Warsaw/protocols#plasmid_DNA_isolation>Isolation</a> of plasmids from cultures inoculated on previous day pACYC177 + OmpA_omega_.</li>
 
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<li>Control <a href=https://2008.igem.org/wiki/index.php?title=Wiki/Team:Warsaw/protocols#digest>digest</a> of isolated plasmids with EcoRI and PstI (Orange buffer). Gel electrophoresis - no proper clones found.</li>
 
<li>Gel electrophoresis of over night digest (RFP(?????) + pT7_alpha) and <a href="https://2008.igem.org/Wiki/Team:Warsaw/protocols#DNA_isolation_from_agarose_gel">gel-out</a> of proper band 800 bp. </li>
<li>Gel electrophoresis of over night digest (RFP(?????) + pT7_alpha) and <a href="https://2008.igem.org/Wiki/Team:Warsaw/protocols#DNA_isolation_from_agarose_gel">gel-out</a> of proper band 800 bp. </li>
<li><a href="https://2008.igem.org/wiki/index.php?title=Wiki/Team:Warsaw/protocols#ligation">Ligation</a> of isolated DNA fragments: 5kb(?????) (from 30 September) + pT7_alpha.</li>
<li><a href="https://2008.igem.org/wiki/index.php?title=Wiki/Team:Warsaw/protocols#ligation">Ligation</a> of isolated DNA fragments: 5kb(?????) (from 30 September) + pT7_alpha.</li>

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Preparation of pACYC177 + OmpA_omega_

Michał K.

  1. Isolation of plasmids from cultures inoculated on previous day pACYC177 + OmpA_omega_.
  2. Control digest of isolated plasmids with EcoRI and PstI (Orange buffer). Gel electrophoresis - no proper clones found.

Preparation of BioBricks

Michał K.

  1. Gel electrophoresis of over night digest (RFP(?????) + pT7_alpha) and gel-out of proper band 800 bp.
  2. Ligation of isolated DNA fragments: 5kb(?????) (from 30 September) + pT7_alpha.

Piotr

  1. Transformation of TOP10 with overnight ligation - RFP(?????) + AraC+pBAD+AID and 5kb(?????) + pT7_alpha.
  2. Planting: RFP(?????) + AraC+pBAD+AID on LB with ampicillin and 5kb(?????) + pT7_alpha on LB with kanamycin.

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