Team:Brown/Notebook/Timeline

From 2008.igem.org

(Difference between revisions)
(New page: {{BrownTemp}} *Team Toxipop created a timeline back in May to follow throughout the summer. What actually happened compared to what we had planned on the timeline were two different stor...)
Line 1: Line 1:
{{BrownTemp}}
{{BrownTemp}}
-
*Team Toxipop created a timeline back in May to follow throughout the summer.  What actually happened compared to what we had planned on the timeline were two different stories, however.
+
*Team Toxipop created a timeline back in May to follow throughout the summer.   
 +
 
 +
====Timeline as presented to Faculty Mentors and Graduate Advisors in May:====
 +
 
 +
* Week 1: Obtaining DNA from Texas lab or outsourcing production. Run experiments testing known methods of lysis & resistance measurements—change procedure and apparatus as necessary.
 +
 
 +
* Week 2: Insert S-R-Rz cassette into plasmid and transform into bacteria.  Screen bacteria to verify transformation success.
 +
 
 +
* Week 3: Create testing apparatus for resistance measurement.  Begin modeling.
 +
 
 +
* Weeks 4-7: Experimentation
 +
 
 +
* Week 8: Analysis of Data, Graphing, Presentation
 +
 
 +
* Weeks 9 - 10: Allowance for any delays, further experimentation, begin second project.
 +
 
 +
====Actual Timeline====
 +
 
 +
*We were able to get the multiple strains from Vivek Jain and John Mekalanos at the Harvard Medical School. 
 +
1 pRG1 DHalpha cells containing the S,R,Rz lysis cassette under a Plac promoter
 +
2 pDKL02 S17-1 cells with the lysis cassette under an IPTG promoter (also containing the ''mob'' element)
 +
3 '''pVJ4 SM10 cells.  Lysis cassette from RY100 cloned into pBAD18.mob 1.'''

Revision as of 01:03, 27 October 2008



  • Team Toxipop created a timeline back in May to follow throughout the summer.

Timeline as presented to Faculty Mentors and Graduate Advisors in May:

  • Week 1: Obtaining DNA from Texas lab or outsourcing production. Run experiments testing known methods of lysis & resistance measurements—change procedure and apparatus as necessary.
  • Week 2: Insert S-R-Rz cassette into plasmid and transform into bacteria. Screen bacteria to verify transformation success.
  • Week 3: Create testing apparatus for resistance measurement. Begin modeling.
  • Weeks 4-7: Experimentation
  • Week 8: Analysis of Data, Graphing, Presentation
  • Weeks 9 - 10: Allowance for any delays, further experimentation, begin second project.

Actual Timeline

  • We were able to get the multiple strains from Vivek Jain and John Mekalanos at the Harvard Medical School.

1 pRG1 DHalpha cells containing the S,R,Rz lysis cassette under a Plac promoter 2 pDKL02 S17-1 cells with the lysis cassette under an IPTG promoter (also containing the mob element) 3 pVJ4 SM10 cells. Lysis cassette from RY100 cloned into pBAD18.mob 1.

Retrieved from "http://2008.igem.org/Team:Brown/Notebook/Timeline"