Team:Brown/Notebook/Timeline

• Team Toxipop created a timeline back in May to follow throughout the summer.

Timeline as presented to Faculty Mentors and Graduate Advisors in May:

• Week 1: Obtaining DNA from Texas lab or outsourcing production. Run experiments testing known methods of lysis & resistance measurements—change procedure and apparatus as necessary.

• Week 2: Insert S-R-Rz cassette into plasmid and transform into bacteria. Screen bacteria to verify transformation success.

• Week 3: Create testing apparatus for resistance measurement. Begin modeling.

• Weeks 4-7: Experimentation

• Week 8: Analysis of Data, Graphing, Presentation

• Weeks 9 - 10: Allowance for any delays, further experimentation, begin second project.

Actual Timeline

• We were able to get the multiple strains from Vivek Jain and John Mekalanos at the Harvard Medical School.
• 1 pRG1 DH5alpha cells containing the S,R,Rz lysis cassette under a Plac promoter. Ampicillin Resistance.
• 2 pDKL02 S17-1 cells with the lysis cassette under an IPTG promoter (also containing the mob element). Kanamycin Resistant.
• 3 pVJ4 SM10 cells. Lysis cassette from RY100 cloned into pBAD18.mob 1. Amp Resistant.
• 4 pVJ13 DH5alpha cells clonded into pBAD33. Chloramphenicol Resistant.