Team:University of Washington/Parts

From 2008.igem.org

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{| style="color:Gold;background-color:#500050;border: none" cellpadding="4" cellspacing="5" border="5" width="99%" align="center"
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|You can write a background of your team here. Give us a background of your team, the members, etc.  Or tell us more about something of your choosing.
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!align="center";style="border: none;" |[[Image:WashingtonColorSeal-21-clip.gif|50px]]
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|[[Image:Example_logo.png|200px|right|frame]]
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!align="center"; style="border: #6b0c6a outset 3px;" |[[Team:University_of_Washington|<font color="gold">Home</font>]]
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!align="center"; style="border: #6b0c6a outset 3px;" |[[Team:University_of_Washington/Team|<font color="gold">The Team</font>]]
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!align="center"; style="border: #6b0c6a outset 3px;" |[[Team:University_of_Washington/Project|<font color="gold">The Project</font>]]
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!align="center"; style="border: #6b0c6a outset 3px;" |[[Team:University_of_Washington/Modeling|<font color="gold">Modeling</font>]]
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!align="center"; style="border: #6b0c6a outset 3px;" |[[Team:University_of_Washington/Notebook|<font color="gold">Notebook</font>]]
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!align="center"; style="border: #6b0c6a outset 3px;" |[[Team:University_of_Washington/Protocols|<font color="gold">Protocols</font>]]
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!align="center"; style="border: #6b0c6a inset 3px;" |[[Team:University_of_Washington/Parts|<font color="gold">Parts Submitted <br>to the Registry</font>]]
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!align="center"; style="background-color:#03943d;border: #6b0c6a outset 3px;" |[[Team:University_of_Washington/Measurement Kit|<font color="gold">Measurement Kit</font>]]
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!align="center"; style="background-color:#c42317;border: #6b0c6a outset 3px;" |[[Team:University_of_Washington/SeToB|<font color="gold">SeToB</font>]]
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!align="center"; style="border: #6b0c6a outset 3px;" |[[Team:University_of_Washington/Safety|<font color="gold">Safety</font>]]
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|}
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===Parts===
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Sent:
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{| style="text-align:center;color:Black;background-color:Gold;border-color:#6b0c6a;" border=2 cellpadding=5  cellspacing=2
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!Parts!!Vector plasmid!!Resistance!!Description
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''Tell us more about your project.  Give us background.  Use this is the abstract of your project.  Be descriptive but concise (1-2 paragraphs)''
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|[[Image:Team.png|right|frame|Your team picture]]
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|-
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|BBa_K109200||pSB1AC3||amp/kan||align="left"|Promoter from Michael Elowitz’s group activatable by AraC/arabinose and repressible by TetR.
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|align="center"|[[Team:University_of_Washington | Team Example 2]]
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|-
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|BBa_K109201||pSB3K3||kan||align="left"|K109201 promoter in front of E0240 GFP
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|-
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|BBa_K109202||pSB3K3||kan||align="left"|K109201 promoter in front of LuxR (I0462)
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|-
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|BBa_K109550||pSB1A3||amp||align="left"|Conjugative master regulator KorA
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|-
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|BBa_K109551||pSB1A3||amp||align="left"|Conjugative factor TrbA
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|-
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|BBa_K109702||pSB3K3||kan||align="left"|Lac promoter (R0010) in front of LuxR (I0462)
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<!--- The Mission, Experiments --->
 
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{| style="color:Yellow;background-color:#0b0c0a;border-color:#6b0c6a;" cellpadding="4" cellspacing="5" border="5" width="73%" align="center"
 
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!align="center"; style="border-color:#6b0c6a"|[[Team:University_of_Washington|Home]]
 
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!align="center"; style="border-color:#6b0c6a"|[[Team:University_of_Washington/Team|The Team]]
 
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!align="center"; style="border-color:#6b0c6a"|[[Team:University_of_Washington/Project|The Project]]
 
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!align="center"; style="border-color:#6b0c6a;background-color:#500050"|[[Team:University_of_Washington/Parts|Parts Submitted to the Registry]]
 
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!align="center"; style="border-color:#6b0c6a"|[[Team:University_of_Washington/Modeling|Modeling]]
 
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!align="center"; style="border-color:#6b0c6a"|[[Team:University_of_Washington/Notebook|Notebook]]
 
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|}
 
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On process:
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{| style="text-align:left;color:Black;background-color:Gold;border-color:#6b0c6a;" border=2 cellpadding=5  cellspacing=2
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===Note===
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|-
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!align="center"|Name!!align="center"|Description
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If you choose to include a '''Parts Submitted to the Registry''' page, please list your parts here.  This is not necessary but it may be a nice list to keep track of.
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|-
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|K109000||pAC88, yeast shuttle vector, nonstandard format
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|-
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|K109001||pAC99, yeast shuttle vector with LAC insert, nonstandard format
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|-
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|K109002||CEN6-ARS4, yeast origin of replication
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|-
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|K109003||HIS3, yeast selectable marker
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|-
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|K109004||RP4 OriT, origin of transfer
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|-
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|K109005||yeast cloning vector (base vector I51020, yeast origin of replication, and yeast selectable marker)
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|-
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|K109006||yeast shuttle vector (base vector I51020, yeast origin of replication, yeast selectable marker, origin of transfer, bacterial origin of replication, and bacterial selectable marker)
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|-
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|K109050||RBS measurement construct w/ GFP (J61100 + E0040)
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|-
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|K109051||RBS measurement construct w/ GFP (J61101 + E0040)
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|-
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|K109052||RBS measurement construct w/ GFP (J61102 + E0040)
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|-
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|K109053||RBS measurement construct w/ GFP (J61103 + E0040)
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|-
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|K109054||RBS measurement construct w/ GFP (J61104 + E0040)
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|-
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|K109060||RBS measurement construct w/ mRFP (J61100 + E1010)
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|-
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|K109061||RBS measurement construct w/ mRFP (J61101 + E1010)
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|-
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|K109062||RBS measurement construct w/ mRFP (J61102 + E1010)
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|-
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|K109063||RBS measurement construct w/ mRFP (J61103 + E1010)
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|-
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|K109064||RBS measurement construct w/ mRFP (J61104 + E1010)
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|}

Latest revision as of 07:54, 27 October 2008

WashingtonColorSeal-21-clip.gif Home The Team The Project Modeling Notebook Protocols Parts Submitted
to the Registry
Measurement Kit SeToB Safety

Parts

Sent:

PartsVector plasmidResistanceDescription
BBa_K109200pSB1AC3amp/kanPromoter from Michael Elowitz’s group activatable by AraC/arabinose and repressible by TetR.
BBa_K109201pSB3K3kanK109201 promoter in front of E0240 GFP
BBa_K109202pSB3K3kanK109201 promoter in front of LuxR (I0462)
BBa_K109550pSB1A3ampConjugative master regulator KorA
BBa_K109551pSB1A3ampConjugative factor TrbA
BBa_K109702pSB3K3kanLac promoter (R0010) in front of LuxR (I0462)


On process:

NameDescription
K109000pAC88, yeast shuttle vector, nonstandard format
K109001pAC99, yeast shuttle vector with LAC insert, nonstandard format
K109002CEN6-ARS4, yeast origin of replication
K109003HIS3, yeast selectable marker
K109004RP4 OriT, origin of transfer
K109005yeast cloning vector (base vector I51020, yeast origin of replication, and yeast selectable marker)
K109006yeast shuttle vector (base vector I51020, yeast origin of replication, yeast selectable marker, origin of transfer, bacterial origin of replication, and bacterial selectable marker)
K109050RBS measurement construct w/ GFP (J61100 + E0040)
K109051RBS measurement construct w/ GFP (J61101 + E0040)
K109052RBS measurement construct w/ GFP (J61102 + E0040)
K109053RBS measurement construct w/ GFP (J61103 + E0040)
K109054RBS measurement construct w/ GFP (J61104 + E0040)
K109060RBS measurement construct w/ mRFP (J61100 + E1010)
K109061RBS measurement construct w/ mRFP (J61101 + E1010)
K109062RBS measurement construct w/ mRFP (J61102 + E1010)
K109063RBS measurement construct w/ mRFP (J61103 + E1010)
K109064RBS measurement construct w/ mRFP (J61104 + E1010)