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EPF-Lausanne/6 October 2008
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| + | ==Cloning== | ||
| + | |||
| + | Results of the plating of RBS-LuxI-Term: | ||
| + | |||
| + | 40 colonies, and 6 on the vector autoligation. | ||
| + | |||
| + | *8 colonies picked and miniprep'd. | ||
| + | *Ctrl. digestion with X,P. | ||
| + | |||
| + | --> image of gel page 106 | ||
| + | |||
| + | Expected bands: | ||
| + | *2.1kb (vector pSB1A2) | ||
| + | *800bp (luxI by 2step pcr) | ||
| + | |||
| + | Clones 2, 3, 5 and 8 are correct. | ||
Revision as of 14:51, 27 October 2008
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Cloning
Results of the plating of RBS-LuxI-Term:
40 colonies, and 6 on the vector autoligation.
- 8 colonies picked and miniprep'd.
- Ctrl. digestion with X,P.
--> image of gel page 106
Expected bands:
- 2.1kb (vector pSB1A2)
- 800bp (luxI by 2step pcr)
Clones 2, 3, 5 and 8 are correct.
