Team:Paris/Modeling/Implementation

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(New page: {{Paris/Menu}} {{Paris/Header|Implementation}} https://2008.igem.org/Team:Paris/Modeling/Workflow_Example Back to "Workflow on an Example" We use '''Matlab''' for all implementations...)
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The experimental datas consist typically in two tables, X_data (various concentrations of the transcription factor) and Y_data (corresponding output values, deduced from the fluorescence of the reporter GFP).  
The experimental datas consist typically in two tables, X_data (various concentrations of the transcription factor) and Y_data (corresponding output values, deduced from the fluorescence of the reporter GFP).  
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* controlling X_data : thanks to the prior characterization of the inductible promoters that control the transcription factor concentrations, we can deduce from the Inv_f1.m and Inv_f2.m functions the necessary concentrations of ''aTc'' and ''arabinose'' to introduce in the medium to get the wanted concentrations of transcription factor.
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* controlling X_data : thanks to the prior characterization of the inductible promoters that control the transcription factor concentrations, we can deduce from the <span style="color:#0000FF;">Inv_f1.m</span> and <span style="color:#0000FF;">Inv_f2.m</span> functions the necessary concentrations of ''aTc'' and ''arabinose'' to introduce in the medium to get the wanted concentrations of transcription factor.
* getting Y_data : the linear conversion between the fluorescence of GFP at maturation and its concentration gives us directly the expected datas.
* getting Y_data : the linear conversion between the fluorescence of GFP at maturation and its concentration gives us directly the expected datas.

Revision as of 18:33, 27 October 2008

Implementation


[Back to "Workflow on an Example"]

We use Matlab for all implementations.

Parameters Finder Programs

The experimental datas consist typically in two tables, X_data (various concentrations of the transcription factor) and Y_data (corresponding output values, deduced from the fluorescence of the reporter GFP).

  • controlling X_data : thanks to the prior characterization of the inductible promoters that control the transcription factor concentrations, we can deduce from the Inv_f1.m and Inv_f2.m functions the necessary concentrations of aTc and arabinose to introduce in the medium to get the wanted concentrations of transcription factor.
  • getting Y_data : the linear conversion between the fluorescence of GFP at maturation and its concentration gives us directly the expected datas.
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