Team:Warsaw/Calendar-Main/27 September 2008

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<h3>Mutagenesis of protein A</h3><h4>Paweł</h4>
<h3>Mutagenesis of protein A</h3><h4>Paweł</h4>
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<p><ol><li>Isolation of plasmid DNA from overnight cultures (it was really tough work...).</li>
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<p><ol><li>Isolation of plasmid DNA from overnight cultures (it was really hard work...).</li>
<li>Control digest of isolated plasmids with KpnI and BamHI (if mutagenesis really occured, ~550bp band should be visible).</li>
<li>Control digest of isolated plasmids with KpnI and BamHI (if mutagenesis really occured, ~550bp band should be visible).</li>
<li>Results of digest:</li>
<li>Results of digest:</li>

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Mutagenesis of protein A

Paweł

  1. Isolation of plasmid DNA from overnight cultures (it was really hard work...).
  2. Control digest of isolated plasmids with KpnI and BamHI (if mutagenesis really occured, ~550bp band should be visible).
  3. Results of digest:
  4. Plasmids which gave positive digest results prepared for sequencing (one sample from each variant which gave positive result).

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