Alberta NINT/4 June 2008
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[[Team:Alberta_NINT/Notebook | < Back to notebook]] | [[Team:Alberta_NINT/Notebook | < Back to notebook]] | ||
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+ | [[Alberta_NINT/3_June_2008 | < Previous entry]] | ||
lab work (SD): | lab work (SD): | ||
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Digested K102001 with SpeI and PstI. | Digested K102001 with SpeI and PstI. | ||
Ran gel electrophoresis and cut out desired band ~2500bp | Ran gel electrophoresis and cut out desired band ~2500bp | ||
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+ | [[Alberta_NINT/5_June_2008 | Next entry >]] |
Revision as of 21:31, 19 June 2008
lab work (SD):
Transformed XL1-B cells with plasmids K102008, K102009 and negative controls.
lab work (JD):
From sequencing results K102001.1 becomes the K102001. Digested K102001 with SpeI and PstI. Ran gel electrophoresis and cut out desired band ~2500bp