"
Alberta NINT/6 June 2008
From 2008.igem.org
(Difference between revisions)
| Line 1: | Line 1: | ||
[[Team:Alberta_NINT/Notebook | < Back to notebook]] | [[Team:Alberta_NINT/Notebook | < Back to notebook]] | ||
| + | |||
| + | [[Alberta_NINT/5_June_2008 | < Previous entry]] | ||
lab work (JD): | lab work (JD): | ||
| Line 14: | Line 16: | ||
<br><br> | <br><br> | ||
Transformed XL1-B cells with K102008 and K102008 negative control from 05/06/08 ligation. | Transformed XL1-B cells with K102008 and K102008 negative control from 05/06/08 ligation. | ||
| + | |||
| + | [[Alberta_NINT/9_June_2008 | Next entry >]] | ||
Revision as of 21:33, 19 June 2008
lab work (JD):
Not too many colonies found on K102003 and K102005 plates today so carried out ligation again but
increased the volume of plasmid and inserts in my ligation mixes.
Transformed bacteria again with K102003 and K102005 and plated the cells.
Left plates from yesterday in the incubator for the weekend.
lab work (SD):
Isolated DNA from LB + amp50 culture tubes (05/06/08) using QIAprep standard miniprep protocol.
Set up DNA for sequencing.
Transformed XL1-B cells with K102008 and K102008 negative control from 05/06/08 ligation.
