Team:Warsaw/Calendar-Main/17 October 2008

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<li> <a href="https://2008.igem.org/wiki/index.php?title=Wiki/Team:Warsaw/protocols#chemotransform">Transformation</a> of <a href="https://2008.igem.org/Wiki/Team:Warsaw/igem_notebook.htm#top10">TOP10</a> with above ligation.</li>
<li> <a href="https://2008.igem.org/wiki/index.php?title=Wiki/Team:Warsaw/protocols#chemotransform">Transformation</a> of <a href="https://2008.igem.org/Wiki/Team:Warsaw/igem_notebook.htm#top10">TOP10</a> with above ligation.</li>
<li> Plating on LB with kanamycin.</li></ol>
<li> Plating on LB with kanamycin.</li></ol>
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<a name="fig1"><img src="https://static.igem.org/mediawiki/2008/e/e5/Go_17_10_2008.jpg"></a>
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<var><b>Fig. 11.</b>Go_17_10_2008.jpg </var>

Revision as of 23:42, 27 October 2008

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Preparation of OmpA-linker-omega-linker (BBa_K103016)

Michał K.

  1. Isolation of plasmids from cultures inoculated on previous day pACYC177 + OmpA-linker-omega-linker (BBa_K103016).
  2. Control digest of isolated plasmids with EcoRI and PstI (Orange buffer). Gel electrophoresis - no proper clones found.

Preparation of alpha_linker under PT7 (BBa_K103019)

Michał K., Piotr

  1. Gel electrophoresis of over night digest (pSB1A3 carrying alpha_linker under PT7 (BBa_K103019)) and gel-out of proper band - 800 bp.
  2. Ligation of isolated DNA fragments: pSB2K3 (from 30 September) + alpha_linker under PT7 (BBa_K103019).
  3. Transformation of TOP10 with above ligation.
  4. Plating on LB with kanamycin.
Fig. 11.Go_17_10_2008.jpg

Preparation of AID under pBAD/araC (BBa_K103002)

Piotr

  1. Transformation of TOP10 with overnight ligation - pSB1A3 + AID under pBAD/araC (BBa_K103002).
  2. Plating on LB with ampicillin.

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