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Team:Heidelberg/Notebook/Killing II/12thweek
From 2008.igem.org
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Revision as of 00:17, 28 October 2008
12th week
Contents |
Monday 10/20/2008
pSB1A3-Receiver-Colicin cloning
- Minipreps of colE1/E9/E9lys-Receiver with QiaCube, Qiagen
- Send probes to GATC for sequencing
Sender cloning: constitutive promotor-sender
- Minipreps of J23107-Receiver with QiaCube, Qiagen
- Send probe to GATC for sequencing
Tuesday 10/21/2008
Sequencing results of ready cloned BioBrick parts
| EcoRI-site mutation | PstI-site 1 mutation | PstI-site 2 mutation | PstI-site 3 mutation | Prefix | Suffix | complete sequence | |
|---|---|---|---|---|---|---|---|
| colE1_BB_57-1 | + | + | + | + | + | + | + |
| colE1_BB_57-2 | + | + | + | + | + | + | + |
| colE1_BB_57+2 | ? | + | + | ? | + | ? | missing sequence |
| colE1_BB_67+1 | + | + | + | + | + | ? | sequencing failure |
| colE9_BB_(2) | + | XXX | XXX | XXX | + | + | + |
| colE9lys_BB | XXX | XXX | XXX | XXX | + | + | + |
| sender_BB | XXX | XXX | XXX | XXX | + | + | + |
--> colE1_BB_57-1, colE9_BB_(2), colE9lys_BB and sender_BB gave positive sequencing results in all criteria and will be sent to the registry
pSB1A2-Receiver-Colicin cloning
HisTag cloning of Colicins for purification
Sender cloning: pBAD-sender
Sender cloning: constitutive promotor-sender
Wednesday 10/22/2008
pSB1A3-Receiver-Colicin cloning
HisTag cloning of Colicins for purification
Thursday 10/23/2008
pSB1A2-Receiver-Colicin cloning
HisTag cloning of Colicins for purification
Colicin activity test
Friday 10/24/2008
pSB1A3-Receiver-Colicin cloning
HisTag cloning of Colicins for purification
Sender Cloning: pBAD - sender
Sender Cloning: constitutive promotor - sender
Saturday 10/25/2008
pSB1A3-Receiver-Colicin cloning
HisTag cloning of Colicins for purification
Sender Cloning: pBAD - sender
Sender Cloning: constitutive promotor - sender
Sunday 10/26/2008
Theoretical work for documentation.
