Team:NTU-Singapore/Notebook/2 June 2008

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=Monday 2nd June=
=Monday 2nd June=
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*Hung([[User:Greenbear|Greenbear]]), Zhen Fu
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{|border="1" style="background-color:#ffffcc;" cellpadding="20"
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===Personnel===
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Hung([[User:Greenbear|Greenbear]]), Zhen Fu
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===Experiment===
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**Grow BW and W3110 E coli with CoE7 plasmid on agar plates:
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**#1 LBA plate, half for BW, half for W3110 (should have no bacteria growth)
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**#1 LB plate, half for BW, half for W3110
**Amplification of LacI-GFP ([http://partsregistry.org/wiki/index.php?title=Part:BBa_J04430 BBa_J04430]) and Terminator ([http://partsregistry.org/wiki/index.php?title=Part:BBa_B0015 BBa_B0015]) parts.
**Amplification of LacI-GFP ([http://partsregistry.org/wiki/index.php?title=Part:BBa_J04430 BBa_J04430]) and Terminator ([http://partsregistry.org/wiki/index.php?title=Part:BBa_B0015 BBa_B0015]) parts.
**Overnight incubation at 37 degrees C of these 3 agar plates:
**Overnight incubation at 37 degrees C of these 3 agar plates:
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**#LB AmpR spread with 125ul cells with Terminator plasmid.
**#LB AmpR spread with 125ul cells with Terminator plasmid.
**#LB KanR spread with 125ul cells with Terminator plasmid.
**#LB KanR spread with 125ul cells with Terminator plasmid.
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**Inoculation of bacteria containing Colicin E7 plasmid (without AmpR) received from Taiwan. We inoculated the cells in 4 cryotubes, which were then put into 37 degrees incubator for overnight incubation:
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**#2 tubes each containing 5ml LB broth with Ampicilin. (which we expect not to see any bacteria in the next morning)
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**#2 tubes each containing 5ml LB broth without Ampicilin.
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Latest revision as of 00:27, 28 October 2008

Monday 2nd June

Personnel

Hung(Greenbear), Zhen Fu

Experiment

    • Grow BW and W3110 E coli with CoE7 plasmid on agar plates:
      1. 1 LBA plate, half for BW, half for W3110 (should have no bacteria growth)
      2. 1 LB plate, half for BW, half for W3110
    • Amplification of LacI-GFP ([http://partsregistry.org/wiki/index.php?title=Part:BBa_J04430 BBa_J04430]) and Terminator ([http://partsregistry.org/wiki/index.php?title=Part:BBa_B0015 BBa_B0015]) parts.
    • Overnight incubation at 37 degrees C of these 3 agar plates:
      1. LB AmpR spread with 250ul cells with LacI-GFP plasmid.
      2. LB AmpR spread with 125ul cells with Terminator plasmid.
      3. LB KanR spread with 125ul cells with Terminator plasmid.
    • Inoculation of bacteria containing Colicin E7 plasmid (without AmpR) received from Taiwan. We inoculated the cells in 4 cryotubes, which were then put into 37 degrees incubator for overnight incubation:
      1. 2 tubes each containing 5ml LB broth with Ampicilin. (which we expect not to see any bacteria in the next morning)
      2. 2 tubes each containing 5ml LB broth without Ampicilin.
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