Team:NTU-Singapore/Notebook/2 June 2008

From 2008.igem.org

(Difference between revisions)
(Monday 2nd June)
 
(2 intermediate revisions not shown)
Line 3: Line 3:
<div id="header">{{User:Greenbear/sandbox/header}}</div>
<div id="header">{{User:Greenbear/sandbox/header}}</div>
-
<div id="maincontent" style="margin-top:100px;">
+
<div id="maincontent" style="margin-top:130px;">
<html>
<html>
<div id="arrow">
<div id="arrow">
Line 19: Line 19:
{|border="1" style="background-color:#ffffcc;" cellpadding="20"
{|border="1" style="background-color:#ffffcc;" cellpadding="20"
|
|
-
===Hung([[User:Greenbear|Greenbear]]), Zhen Fu===
+
===Personnel===
 +
Hung([[User:Greenbear|Greenbear]]), Zhen Fu
 +
===Experiment===
**Grow BW and W3110 E coli with CoE7 plasmid on agar plates:
**Grow BW and W3110 E coli with CoE7 plasmid on agar plates:
**#1 LBA plate, half for BW, half for W3110 (should have no bacteria growth)
**#1 LBA plate, half for BW, half for W3110 (should have no bacteria growth)

Latest revision as of 00:27, 28 October 2008

Monday 2nd June

Personnel

Hung(Greenbear), Zhen Fu

Experiment

    • Grow BW and W3110 E coli with CoE7 plasmid on agar plates:
      1. 1 LBA plate, half for BW, half for W3110 (should have no bacteria growth)
      2. 1 LB plate, half for BW, half for W3110
    • Amplification of LacI-GFP ([http://partsregistry.org/wiki/index.php?title=Part:BBa_J04430 BBa_J04430]) and Terminator ([http://partsregistry.org/wiki/index.php?title=Part:BBa_B0015 BBa_B0015]) parts.
    • Overnight incubation at 37 degrees C of these 3 agar plates:
      1. LB AmpR spread with 250ul cells with LacI-GFP plasmid.
      2. LB AmpR spread with 125ul cells with Terminator plasmid.
      3. LB KanR spread with 125ul cells with Terminator plasmid.
    • Inoculation of bacteria containing Colicin E7 plasmid (without AmpR) received from Taiwan. We inoculated the cells in 4 cryotubes, which were then put into 37 degrees incubator for overnight incubation:
      1. 2 tubes each containing 5ml LB broth with Ampicilin. (which we expect not to see any bacteria in the next morning)
      2. 2 tubes each containing 5ml LB broth without Ampicilin.