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Alberta NINT/20 June 2008
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(Difference between revisions)
(New page: lab work (SD): Digested K102002.1-.6 and freshly annealed TA0 with BamHI + NcoI. Incubated at 37 C for 2 hrs. Ran K102002.1-.6 on 2% agarose gel at 1...) |
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lab work (SD): | lab work (SD): | ||
Digested K102002.1-.6 and freshly annealed TA0 with BamHI + NcoI. | Digested K102002.1-.6 and freshly annealed TA0 with BamHI + NcoI. | ||
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Quantified DNA for K102002 and TA0. | Quantified DNA for K102002 and TA0. | ||
Ligated TA0 into K102002.1-.6 and incubated at 16 C for 30 min. | Ligated TA0 into K102002.1-.6 and incubated at 16 C for 30 min. | ||
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| + | [[Alberta_NINT/21_June_2008 | Next entry >]] | ||
Revision as of 17:21, 20 June 2008
lab work (SD):
Digested K102002.1-.6 and freshly annealed TA0 with BamHI + NcoI.
Incubated at 37 C for 2 hrs.
Ran K102002.1-.6 on 2% agarose gel at 110V for 50 min.
Purified TA0 using QIAquick nucleotide removal protocol.
Quantified DNA for K102002 and TA0.
Ligated TA0 into K102002.1-.6 and incubated at 16 C for 30 min.
